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Literature summary extracted from

  • Iijima, N.; Uchiyama, S.; Fujikawa, Y.; Esaka, M.
    Purification, characterization, and molecular cloning of group I phospholipases A2 from the gills of the red sea bream, Pagrus major (2000), Lipids, 35, 1359-1370.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.4 sodium cholate stimulates, optimal concentration: 6-8 mM Pagrus major

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 isoform G-3 PLA2 Pagrus major

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 EDTA complete inhibition at 1 mM Pagrus major
3.1.1.4 EGTA complete inhibition at 1 mM Pagrus major
3.1.1.4 Sodium dodecyl sulfate
-
 Pagrus major

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+ requirement, optimal concentration: 2-40 mM Pagrus major

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 15000
-
 SDS-PAGE Pagrus major

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Pagrus major
-
 red sea bream
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4 isoforms G-1 PLA2, G-1 PLA2, G-3 PLA2 Pagrus major

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 adipose tissue
-
 Pagrus major
-
3.1.1.4 gill highest activity Pagrus major
-
3.1.1.4 heart
-
 Pagrus major
-
3.1.1.4 hepatopancreas
-
 Pagrus major
-
3.1.1.4 intestine
-
 Pagrus major
-
3.1.1.4 testis
-
 Pagrus major
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 1143
-
 isoform G-2 PLA2 Pagrus major
3.1.1.4 1293
-
 isoform G-3 PLA2 Pagrus major
3.1.1.4 1294
-
 isoform G-1 PLA2 Pagrus major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O
-
 Pagrus major 1-palmitoyl-sn-glycero-3-phosphocholine + oleic acid
-
 ?
3.1.1.4 phosphatidylcholine + H2O
-
 Pagrus major 1-acylglycerophosphocholine + fatty acid
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 9 10
-
 Pagrus major