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Literature summary extracted from

  • Chroni, A.; Mavri-Vavayanni, M.
    Characterization of a platelet activating factor acetylhydrolase from rat adipocyte (2000), Life Sci., 67, 2807-2825.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.47 dithiothreitol slightly acivating Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.47 Ca2+ 18% inhibition at 10 mM Rattus norvegicus
3.1.1.47 DTNB 69% inhibition at 2 mM Rattus norvegicus
3.1.1.47 lyso-2-hexadecyl-1-alkyl-sn-glycero-3-phosphocholine 22% inhibition at 0.05 mM Rattus norvegicus
3.1.1.47 additional information no inhibition by EGTA at 10 mM and acetylsalicylic acid at 0.2 mM Rattus norvegicus
3.1.1.47 phosphocholine 25% inhibition at 0.05 mM Rattus norvegicus
3.1.1.47 PMSF 93% inhibition at 2 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.47 0.0049
-
 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine pH 7.2, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.47 cytosol adipocyte Rattus norvegicus 5829
-
3.1.1.47 additional information activity in subcellular fractions Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.47 37000
-
 x * 66000, major part, + x * 37000, mino part, SDS-PAGE Rattus norvegicus
3.1.1.47 66000
-
 x * 66000, major part, + x * 37000, mino part, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.47 2-hexadecyl-1-alkyl-sn-glycero-3-phosphocholine + H2O Rattus norvegicus i.e. platelet-activation factor, substrate and enzyme play important roles in inflammation processes 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.47 Rattus norvegicus
-
 male Wistar rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.47 from adipocyte, 45.9fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.47 adipocyte
-
 Rattus norvegicus
-
3.1.1.47 epididymis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.47 0.02
-
 partially purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.47 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O
-
 Rattus norvegicus 1-alkyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor Rattus norvegicus 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 2-hexadecyl-1-alkyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor, substrate and enzyme play important roles in inflammation processes Rattus norvegicus 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.47 ? x * 66000, major part, + x * 37000, mino part, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.47 PAF-AH
-
 Rattus norvegicus
3.1.1.47 platelet activating factor acetylhydrolase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.47 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.47 37
-
 30 min, stable Rattus norvegicus
3.1.1.47 60
-
 10 min, almost complete inactivation Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.47 7.4
-
 broad optimum Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.47 5 7.4 inactivation below pH 5.0, active to mild alkaline range Rattus norvegicus