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Literature summary extracted from

  • Choi, Y.M.; Suh, H.J.; Kim, J.M.
    Purification and properties of extracellular phytase from Bacillus sp. KHU-10 (2001), J. Protein Chem., 20, 287-292.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.3.8 CaCl2 increases pH-stability and thermal stability Bacillus sp. (in: Bacteria)
3.1.3.26 CaCl2 increases pH-stability and thermal stability Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Ba2+
-
Bacillus sp. (in: Bacteria)
3.1.3.8 Cd2+
-
Bacillus sp. (in: Bacteria)
3.1.3.8 Co2+
-
Bacillus sp. (in: Bacteria)
3.1.3.8 Cr3+
-
Bacillus sp. (in: Bacteria)
3.1.3.8 Cu2+
-
Bacillus sp. (in: Bacteria)
3.1.3.8 EDTA 1 mM, 94% inhibition Bacillus sp. (in: Bacteria)
3.1.3.8 Hg2+
-
Bacillus sp. (in: Bacteria)
3.1.3.8 Mn2+
-
Bacillus sp. (in: Bacteria)
3.1.3.26 Ba2+
-
Bacillus sp. (in: Bacteria)
3.1.3.26 Cd2+
-
Bacillus sp. (in: Bacteria)
3.1.3.26 Co2+
-
Bacillus sp. (in: Bacteria)
3.1.3.26 Cr3+
-
Bacillus sp. (in: Bacteria)
3.1.3.26 Cu2+
-
Bacillus sp. (in: Bacteria)
3.1.3.26 EDTA 1 mM, 94% inhibition Bacillus sp. (in: Bacteria)
3.1.3.26 Hg2+
-
Bacillus sp. (in: Bacteria)
3.1.3.26 Mn2+
-
Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.05
-
myo-inositol hexakisphosphate pH 7.0 Bacillus sp. (in: Bacteria)
3.1.3.26 0.05
-
myo-inositol hexakisphosphate pH 7.0 Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 extracellular
-
Bacillus sp. (in: Bacteria)
-
-
3.1.3.26 extracellular
-
Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 44000
-
1 * 44000, SDS-PAGE Bacillus sp. (in: Bacteria)
3.1.3.8 46000
-
gel filtration Bacillus sp. (in: Bacteria)
3.1.3.26 44000
-
1 * 44000, SDS-PAGE Bacillus sp. (in: Bacteria)
3.1.3.26 46000
-
gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Bacillus sp. (in: Bacteria)
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Bacillus sp. (in: Bacteria) KHU-10
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Bacillus sp. (in: Bacteria)
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Bacillus sp. (in: Bacteria) KHU-10
-
The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
Bacillus sp. (in: Bacteria)
3.1.3.26
-
Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.8 cell culture phytase increases markedly at the late stationary phase Bacillus sp. (in: Bacteria)
-
3.1.3.26 cell culture phytase increases markedly at the late stationary phase Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 36
-
-
Bacillus sp. (in: Bacteria)
3.1.3.26 36
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 additional information no activity with 4-nitrophenyl phosphate, beta-glycerophosphate, glucose 6-phosphate, sodium glycerophosphate, AMP, ADP and ATP Bacillus sp. (in: Bacteria) ?
-
?
3.1.3.8 additional information no activity with 4-nitrophenyl phosphate, beta-glycerophosphate, glucose 6-phosphate, sodium glycerophosphate, AMP, ADP and ATP Bacillus sp. (in: Bacteria) KHU-10 ?
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O very specific for, no activity on other phosphate esters Bacillus sp. (in: Bacteria) ? + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O very specific for, no activity on other phosphate esters Bacillus sp. (in: Bacteria) KHU-10 ? + phosphate
-
?
3.1.3.26 additional information no activity with 4-nitrophenyl phosphate, beta-glycerophosphate, glucose 6-phosphate, sodium glycerophosphate, AMP, ADP and ATP Bacillus sp. (in: Bacteria) ?
-
?
3.1.3.26 additional information no activity with 4-nitrophenyl phosphate, beta-glycerophosphate, glucose 6-phosphate, sodium glycerophosphate, AMP, ADP and ATP Bacillus sp. (in: Bacteria) KHU-10 ?
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O very specific for, no activity on other phosphate esters Bacillus sp. (in: Bacteria) ? + phosphate
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O very specific for, no activity on other phosphate esters Bacillus sp. (in: Bacteria) KHU-10 ? + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 monomer 1 * 44000, SDS-PAGE Bacillus sp. (in: Bacteria)
3.1.3.26 monomer 1 * 44000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 40
-
without Ca2+ Bacillus sp. (in: Bacteria)
3.1.3.8 60
-
in presence of 10 mM CaCl2 Bacillus sp. (in: Bacteria)
3.1.3.26 40
-
without Ca2+ Bacillus sp. (in: Bacteria)
3.1.3.26 60
-
in presence of 10 mM CaCl2 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 30 80 30°C: about 40% of maximal activity, 80°C: about 55% of maximal activity, presence of 10 mM CaCl2 Bacillus sp. (in: Bacteria)
3.1.3.26 30 80 30°C: about 40% of maximal activity, 80°C: about 55% of maximal activity, presence of 10 mM CaCl2 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 60
-
10 min, in presence of 10 mM CaCl2, stable up to Bacillus sp. (in: Bacteria)
3.1.3.8 70
-
10 min, in presence of 10 mM CaCl2, 35% loss of activity Bacillus sp. (in: Bacteria)
3.1.3.8 80
-
10 min, in presence of 10 mM CaCl2, 50% loss of activity Bacillus sp. (in: Bacteria)
3.1.3.26 60
-
10 min, in presence of 10 mM CaCl2, stable up to Bacillus sp. (in: Bacteria)
3.1.3.26 70
-
10 min, in presence of 10 mM CaCl2, 35% loss of activity Bacillus sp. (in: Bacteria)
3.1.3.26 80
-
10 min, in presence of 10 mM CaCl2, 50% loss of activity Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 26.6
-
myo-inositol hexakisphosphate pH 7.0 Bacillus sp. (in: Bacteria)
3.1.3.26 26.6
-
myo-inositol hexakisphosphate pH 7.0 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 6 9.5 in presence of 10 mM CaCl2 Bacillus sp. (in: Bacteria)
3.1.3.8 6.5 8.5 without Ca2+ Bacillus sp. (in: Bacteria)
3.1.3.26 6 9.5 in presence of 10 mM CaCl2 Bacillus sp. (in: Bacteria)
3.1.3.26 6.5 8.5 without Ca2+ Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 5 10 pH 5.0: about 50% of maximal activity, pH 10.0: about 80% of maximal activity, presence of 10 mM CaCl2 Bacillus sp. (in: Bacteria)
3.1.3.26 5 10 pH 5.0: about 50% of maximal activity, pH 10.0: about 80% of maximal activity, presence of 10 mM CaCl2 Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.8 Bacillus sp. (in: Bacteria) isoelectric focusing
-
6.8
3.1.3.26 Bacillus sp. (in: Bacteria) isoelectric focusing
-
6.8