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Literature summary extracted from

  • Al-Saleh, S.S.
    Biochemical characterization of the soluble alkaline phosphatase isolated from the venomous snake W. aegyptia (2002), J. Nat. Toxins, 11, 357-365.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 Caffeine uncompetitive inhibition Walterinnesia aegyptia
3.1.3.1 CN- complete inhibition at 10 mM Walterinnesia aegyptia
3.1.3.1 EDTA
-
 Walterinnesia aegyptia
3.1.3.1 imidazole uncompetitive inhibition Walterinnesia aegyptia
3.1.3.1 L-leucine uncompetitive inhibition Walterinnesia aegyptia
3.1.3.1 L-phenylalanine uncompetitive inhibition Walterinnesia aegyptia
3.1.3.1 MnO4- competitive inhibition Walterinnesia aegyptia
3.1.3.1 phosphate competitive inhibition Walterinnesia aegyptia
3.1.3.1 Zn2+ complete inhibition at 15 mM Walterinnesia aegyptia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.1 extracellular venom Walterinnesia aegyptia
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Zn2+ metalloenzyme Walterinnesia aegyptia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 55000
-
 x * 80000 + x * 55000, SDS-PAGE Walterinnesia aegyptia
3.1.3.1 80000
-
 x * 80000 + x * 55000, SDS-PAGE Walterinnesia aegyptia

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Walterinnesia aegyptia
-
 venomous snake
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1 3.4fold from venom Walterinnesia aegyptia

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.1 a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Walterinnesia aegyptia
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.1 venom
-
 Walterinnesia aegyptia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 24
-
 partially purified enzyme Walterinnesia aegyptia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Walterinnesia aegyptia 4-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 ? x * 80000 + x * 55000, SDS-PAGE Walterinnesia aegyptia

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 alkaline phosphatase
-
 Walterinnesia aegyptia
3.1.3.1 ALP
-
 Walterinnesia aegyptia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 25
-
 assay at Walterinnesia aegyptia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 9.8
-
 assay at Walterinnesia aegyptia

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.1 2
-
 phosphate pH 9.8, 25°C Walterinnesia aegyptia
3.1.3.1 3
-
 L-phenylalanine pH 9.8, 25°C Walterinnesia aegyptia
3.1.3.1 4
-
 MnO4- pH 9.8, 25°C Walterinnesia aegyptia
3.1.3.1 5
-
 imidazole pH 9.8, 25°C Walterinnesia aegyptia
3.1.3.1 5.4
-
 L-leucine pH 9.8, 25°C Walterinnesia aegyptia
3.1.3.1 17.4
-
 Caffeine pH 9.8, 25°C Walterinnesia aegyptia

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.1 Walterinnesia aegyptia isoelectric focusing, 2 bands at pH 6.2 and pH 6.8
-
 6.2
3.1.3.1 Walterinnesia aegyptia isoelectric focusing, 2 bands at pH 6.2 and pH 6.8
-
 6.8