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Literature summary extracted from

  • Sousa, H.A.; Afonso, C.A.M.; Crespo, J.G.
    Kinetic study of the enantioselective hydrolysis of a meso-diester using pig liver Esterase (2000), J. Chem. Technol. Biotechnol., 75, 707-714.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.1 synthesis methyl hydrogen (1S,2R)-cyclohex-4-ene-1,2-dicarboxylate is an important educt for the synthesis of diverse biologically relevant products such as carbacyclin, prostaglandin synthon, brefeldin, anticapsin and others Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 methanol noncompetitive product inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 additional information
-
 additional information kinetics Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Sus scrofa
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.1 a carboxylic ester + H2O = an alcohol + a carboxylate reaction mechanism Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.1 liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 dimethyl cis-cyclohex-4-ene-1,2-dicarboxylate + H2O enantioselectivity, 98% at 25°C, 96% at 50°C Sus scrofa methyl hydrogen (1S,2R)-cyclohex-4-ene-1,2-dicarboxylate + methanol 94-97% of the R-enantiomer ir

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 pig liver esterase
-
 Sus scrofa
3.1.1.1 PLE
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 25
-
-
 Sus scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 25 50
-
 Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 25
-
 good long-term stability Sus scrofa
3.1.1.1 45 50 denaturation occurs Sus scrofa
3.1.1.1 55
-
 rapid denaturation Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 6 8.5
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.1 additional information
-
 additional information
-
 Sus scrofa