EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.78 | D169A | 50% of wild-type activity with 37-mer oligodeoxyribonucleotide substrate, no reverse reaction | Kostyavirus CJW1 |
2.7.1.78 | D175A | 60% of wild-type activity with 37-mer oligodeoxyribonucleotide substrate, 68% of wild-type activity with CMP substrate | Omegavirus omega |
2.7.1.78 | K15A | no enzymic activity in kinase reaction, 58% of wild-type activity in 3-phosphates reaction | Kostyavirus CJW1 |
2.7.1.78 | K16A | no enzymic activity | Omegavirus omega |
3.1.3.32 | D169A | half of wild type 5-kinase activity, almost complete loss of 3-phosphatase activity | Kostyavirus CJW1 |
3.1.3.32 | D175A | reduced 5-kinase activity, no 3-phosphatase activity | Omegavirus omega |
3.1.3.32 | K15A | no 5-kinase activity, reduced 3-phosphates activity | Kostyavirus CJW1 |
3.1.3.32 | K16A | retains 5-kinase activity, reduced 3-phosphatase activity | Omegavirus omega |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.78 | Mg2+ | 1-2 mM, maximum activity, inhibitory above | Kostyavirus CJW1 | |
2.7.1.78 | Mg2+ | 1-2 mM, maximum activity, inhibitory above | Omegavirus omega |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.78 | 0.007 | - |
ATP | pH 6.5, 37°C | Omegavirus omega | |
2.7.1.78 | 0.04 | - |
ATP | pH 6.5, 37°C | Kostyavirus CJW1 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.78 | Co2+ | maximum activity at 0.6-2.5 mM | Kostyavirus CJW1 | |
2.7.1.78 | Mg2+ | 1-2 mM, maximum activity, inhibitory above | Omegavirus omega | |
2.7.1.78 | Mg2+ | about 60% as efficient as Co2+ | Kostyavirus CJW1 | |
2.7.1.78 | Mn2+ | half as effective as Mg2+ | Omegavirus omega | |
2.7.1.78 | Mn2+ | about 60% as efficient as Co2+ | Kostyavirus CJW1 | |
2.7.1.78 | additional information | divalent metal ion required | Omegavirus omega | |
3.1.3.32 | Co2+ | divalent cation required, order of decreasing preference: Co2+, Mg2+, Mn2+ | Kostyavirus CJW1 | |
3.1.3.32 | Co2+ | divalent metal ion required, fulfilled by Co2+, Mg2+, Mn2+ at 10 mM | Omegavirus omega | |
3.1.3.32 | Mg2+ | divalent cation required, order of decreasing preference: Co2+, Mg2+, Mn2+ | Kostyavirus CJW1 | |
3.1.3.32 | Mg2+ | divalent metal ion required, fulfilled by Co2+, Mg2+, Mn2+ at 10 mM | Omegavirus omega | |
3.1.3.32 | Mn2+ | divalent cation required, order of decreasing preference: Co2+, Mg2+, Mn2+ | Kostyavirus CJW1 | |
3.1.3.32 | Mn2+ | divalent metal ion required, fulfilled by Co2+, Mg2+, Mn2+ at 10 mM | Omegavirus omega |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.78 | 39000 | - |
4 * 39000, recombinant protein with His10-tag, calculated, protein is either a stable but asymmetrically shaped homotetramer or a metastable tetramer that is prone to dissociate to a homodimer | Kostyavirus CJW1 |
2.7.1.78 | 40000 | - |
4 * 40000, recombinant protein with His10-tag, SDS-PAGE, protein is either a stable but asymmetrically shaped homotetramer or a metastable tetramer that is prone to dissociate to a homodimer | Omegavirus omega |
2.7.1.78 | 80000 | 160000 | sedimentation equilibrium analysis | Omegavirus omega |
2.7.1.78 | 80000 | 160000 | sedimentaion equilibrium analysis | Kostyavirus CJW1 |
3.1.3.32 | 40000 | - |
4 * 40000, His10-tagged enzyme, SDS-PAGE | Omegavirus omega |
3.1.3.32 | 80000 | 160000 | sedimentation analysis | Omegavirus omega |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.78 | Kostyavirus CJW1 | - |
expression in Escherichia coli with His10-tag | - |
2.7.1.78 | Omegavirus omega | - |
expression in Escherichia coli with His10-tag | - |
3.1.3.32 | Kostyavirus CJW1 | - |
bifunctional enzyme with proximal 5-kinase and disal 3-phosphatase activity | - |
3.1.3.32 | Omegavirus omega | - |
bifunctional enzyme with proximal 5-kinase and disal 3-phosphatase activity | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.32 | 19 | - |
3-phosphatase activity | Omegavirus omega |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.78 | ATP + 3'-CMP | - |
Omegavirus omega | ADP + pCp | - |
? | |
2.7.1.78 | ATP + 3'-CMP | - |
Kostyavirus CJW1 | ADP + pCp | - |
? | |
2.7.1.78 | ATP + 5'-dephospho-DNA | 37-mer oligodeoxyribonucleotide substrate | Omegavirus omega | ADP + 5'-phospho-DNA | - |
r | |
2.7.1.78 | ATP + 5'-dephospho-DNA | 37-mer oligodeoxyribonucleotide substrate | Kostyavirus CJW1 | ADP + 5'-phospho-DNA | - |
r | |
3.1.3.32 | dTMP + H2O | - |
Omegavirus omega | thymidine + phosphate | - |
? | |
3.1.3.32 | dTMP + H2O | - |
Kostyavirus CJW1 | thymidine + phosphate | - |
? | |
3.1.3.32 | phosphorylated DNA ends + H2O | - |
Omegavirus omega | ? | - |
? | |
3.1.3.32 | phosphorylated DNA ends + H2O | - |
Kostyavirus CJW1 | ? | - |
? | |
3.1.3.32 | phosphorylated DNA-ends + H2O | - |
Omegavirus omega | DNA-ends + phosphate | - |
r | |
3.1.3.32 | phosphorylated DNA-ends + H2O | - |
Kostyavirus CJW1 | DNA-ends + phosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.78 | tetramer | 4 * 39000, recombinant protein with His10-tag, calculated, protein is either a stable but asymmetrically shaped homotetramer or a metastable tetramer that is prone to dissociate to a homodimer | Kostyavirus CJW1 |
2.7.1.78 | tetramer | 4 * 40000, recombinant protein with His10-tag, SDS-PAGE, protein is either a stable but asymmetrically shaped homotetramer or a metastable tetramer that is prone to dissociate to a homodimer | Omegavirus omega |
3.1.3.32 | tetramer | 4 * 40000, His10-tagged enzyme, SDS-PAGE | Omegavirus omega |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.32 | PNKP | - |
Omegavirus omega |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.78 | 0.0043 | - |
ATP | pH 6.5, 37°C | Kostyavirus CJW1 | |
2.7.1.78 | 0.008 | - |
3'-CMP | pH 6.5, 37°C | Kostyavirus CJW1 | |
2.7.1.78 | 0.2 | - |
ATP | pH 6.5, 37°C | Omegavirus omega | |
2.7.1.78 | 1.6 | - |
3'-CMP | pH 6.5, 37°C | Omegavirus omega | |
3.1.3.32 | 0.003 | - |
phosphorylated DNA ends | - |
Omegavirus omega | |
3.1.3.32 | 0.0043 | - |
phosphorylated DNA ends | - |
Kostyavirus CJW1 | |
3.1.3.32 | 0.58 | - |
dTMP | - |
Kostyavirus CJW1 | |
3.1.3.32 | 0.63 | - |
dTMP | - |
Omegavirus omega | |
3.1.3.32 | 6.08 | - |
dTMP | - |
Omegavirus omega | |
3.1.3.32 | 6.08 | - |
dTMP | - |
Kostyavirus CJW1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.78 | 5.5 | 7.5 | 3-CMP kinase activit | Kostyavirus CJW1 |
2.7.1.78 | 8.5 | 9.5 | - |
Omegavirus omega |
3.1.3.32 | 5.5 | - |
- |
Omegavirus omega |
3.1.3.32 | 5.5 | - |
- |
Kostyavirus CJW1 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.78 | 6 | - |
50% of maximum activity | Omegavirus omega |
2.7.1.78 | 6 | - |
50% of maximum activity | Kostyavirus CJW1 |