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Literature summary extracted from

  • Meredith, T.C.; Woodard, R.W.
    Escherichia coli YrbH is a D-arabinose 5-phosphate isomerase (2003), J. Biol. Chem., 278, 32771-32777.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.13
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.13 Cd2+ 0.01 mM, complete inhibition Escherichia coli
5.3.1.13 Cu2+
-
 Escherichia coli
5.3.1.13 EDTA
-
 Escherichia coli
5.3.1.13 Hg2+ 0.01 mM, complete inhibition Escherichia coli
5.3.1.13 Ni2+
-
 Escherichia coli
5.3.1.13 Zn2+ 0.01 mM, complete inhibition, IC50: 0.001-0.003 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.13 0.35
-
 D-ribulose 5-phosphate pH 8.5, 37°C Escherichia coli
5.3.1.13 0.61
-
 D-arabinose 5-phosphate pH 8.5, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.13 35000
-
 4 * 35000, SDS-PAGE Escherichia coli
5.3.1.13 122000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.13 D-ribulose 5-phosphate Escherichia coli first enzyme in the biosynthesis of 3-deoxy-D-manno-octulosonate D-arabinose 5-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.13 Escherichia coli
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.13
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.13 246
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
5.3.1.13 -80°C, stable for at least 1 month Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.13 D-ribulose 5-phosphate
-
 Escherichia coli D-arabinose 5-phosphate
-
 r
5.3.1.13 D-ribulose 5-phosphate first enzyme in the biosynthesis of 3-deoxy-D-manno-octulosonate Escherichia coli D-arabinose 5-phosphate
-
 r
5.3.1.13 additional information DL-glyceraldehyde 3-phosphate and D-erythrose 4-phosphate do not serve as alternate substrates Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.13 tetramer 4 * 35000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
5.3.1.13 API
-
 Escherichia coli
5.3.1.13 YrbH
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.13 157
-
 D-arabinose 5-phosphate pH 8.5, 37°C Escherichia coli
5.3.1.13 255
-
 D-ribulose 5-phosphate pH 8.5, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.13 8.4
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.13 7 10 pH 7.0: about 70% of maximal activity, pH 10.0: about 70% of maximal activity Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.3.1.13 0.001 0.003 0.01 mM, complete inhibition, IC50: 0.001-0.003 mM Escherichia coli Zn2+