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Literature summary extracted from

  • Lindermayr, C.; Fliegmann, J.; Ebel, J.
    Deletion of a single amino acid residue from different 4-coumarate:CoA ligases from soybean results in the generation of new substrate specificities (2003), J. Biol. Chem., 278, 2781-2786.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 expression in Escherichia coli as His-tag fusion protein Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.12 E340A 4CL1-mutant, activity almost completely abolished Glycine max
6.2.1.12 G333A 4CL1-mutant, activity almost completely abolished Glycine max
6.2.1.12 G337A 4CL1-mutant, activity almost completely abolished Glycine max
6.2.1.12 G342A 4CL1-mutant, activity almost completely abolished Glycine max
6.2.1.12 I346A 4CL1-mutant, very low activity with all substrates Glycine max
6.2.1.12 L344A 4CL1-mutant, activity almost completely abolished Glycine max
6.2.1.12 M338A 4CL1-mutant, activity almost completely abolished Glycine max
6.2.1.12 M348A 4CL1-mutant, activity almost completely abolished Glycine max
6.2.1.12 additional information construction of hybrid enzymes using different portions of isoforms 4CL1-4CL3, some of the constructed proteins were found to be inactive Glycine max
6.2.1.12 P285A 4CL1-mutant, very low activity with all substrates Glycine max
6.2.1.12 P286A 4CL1-mutant, minor changes in activity Glycine max
6.2.1.12 P343A 4CL1-mutant, very low activity with all substrates Glycine max
6.2.1.12 Q334A 4CL1-mutant, very low activity with all substrates Glycine max
6.2.1.12 T331A 4CL1-mutant, minor changes in activity Glycine max
6.2.1.12 T339A 4CL1-mutant, activity almost completely abolished Glycine max
6.2.1.12 V284A 4CL1-mutant, minor changes in activity Glycine max
6.2.1.12 Y336A 4CL1-mutant, activity almost completely abolished Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.004
-
 ferulate 4CL1 Glycine max
6.2.1.12 0.005
-
 sinapate 4CL1 Glycine max
6.2.1.12 0.009
-
 4-coumarate 4CL3 Glycine max
6.2.1.12 0.013
-
 caffeate 4CL2 Glycine max
6.2.1.12 0.042
-
 4-coumarate 4CL2 Glycine max
6.2.1.12 0.049
-
 3,4-dimethoxycinnamate 4CL1 Glycine max
6.2.1.12 0.05
-
 4-coumarate 4CL1 Glycine max
6.2.1.12 0.05
-
 caffeate 4CL3 Glycine max
6.2.1.12 0.081
-
 caffeate 4CL1 Glycine max
6.2.1.12 0.14
-
 ferulate 4CL2 Glycine max
6.2.1.12 1.1
-
 cinnamate 4CL3 Glycine max
6.2.1.12 1.7
-
 cinnamate 4CL2 Glycine max
6.2.1.12 3.1
-
 ferulate 4CL3 Glycine max
6.2.1.12 4.4
-
 cinnamate 4CL1 Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Glycine max plant phenylpropanoid metabolism AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Glycine max
-
 isoforms 4CL1-4CL3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 recombinant protein using His-tag Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 3,4-dimethoxycinnamate + CoA substrate for 4CL1 but not for 4CL2 and 4CL3 Glycine max AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Glycine max AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA plant phenylpropanoid metabolism Glycine max AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Glycine max AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA
-
 Glycine max AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA substrate for 4CL1 and 4CL2, very poor substrate for 4CL3 Glycine max AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + sinapate + CoA substrate for 4CL1 but not for 4CL2 and 4CL3 Glycine max AMP + diphosphate + sinapoyl-CoA
-
 ?