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Literature summary extracted from
- YMR313c/TGL3 encodes a novel triacylglycerol lipase located in lipid particles of Saccharomyces cerevisiae (2003), J. Biol. Chem., 278, 23317-23323.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | overexpression of His-tagged Tgl13 hybrid in a yeast strain | Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | additional information | deletion mutant strain DELTAtgl13 shows accumulation of triacylglycerols | Saccharomyces cerevisiae |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.3 | lipid particle | exclusively | Saccharomyces cerevisiae | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.3 | triacylglycerol + H2O | Saccharomyces cerevisiae | - |
diacylglycerol + a carboxylate | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.3 | Saccharomyces cerevisiae | - |
gene YMR313c, enzyme Tgl3p | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | recombinant hybrid enzyme, to near homogeneity, 174fold | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 0.071 | - |
lipid particles, wild-type | Saccharomyces cerevisiae |
| 3.1.1.3 | 12.3 | - |
purified recombinant His-tagged enzyme | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.3 | triacylglycerol + H2O | - |
Saccharomyces cerevisiae | diacylglycerol + a carboxylate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | lipase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 8 | - |
assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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