EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.3.2 | DNA sequence determination and analysis, expression in Escherichia coli BL21 as full length or truncated protein, the latter lacking the mitochondrial targeting sequence, functional complementation of enzyme deficient yeast fau1 mutant, yeast strain WHY1.3.1 | Arabidopsis thaliana |
6.3.3.2 | DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) as full length or truncated protein, the latter lacking the mitochondrial targeting sequence | Solanum lycopersicum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.3.3.2 | mitochondrion | exclusively | Pisum sativum | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.3.2 | 26500 | - |
1 * 26500, recombinant truncated enzyme, amino acid sequence determination | Arabidopsis thaliana |
6.3.3.2 | 27000 | - |
recombinant truncated enzyme, gel filtration | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.3.2 | ATP + 5-formyltetrahydrofolate | Pisum sativum | - |
ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? | |
6.3.3.2 | ATP + 5-formyltetrahydrofolate | Arabidopsis thaliana | - |
ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
ir | |
6.3.3.2 | ATP + 5-formyltetrahydrofolate | Solanum lycopersicum | - |
ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.3.2 | Arabidopsis thaliana | Q8L539 | - |
- |
6.3.3.2 | Pisum sativum | - |
- |
- |
6.3.3.2 | Solanum lycopersicum | Q8LKF6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.3.2 | to near homogeneity, recombinant enzyme from Escherichia coli | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.3.3.2 | leaf | high content | Pisum sativum | - |
6.3.3.2 | root | low content | Pisum sativum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.3.2 | additional information | - |
activity of recombinant enzyme lacking the mitochondrial targeting sequence is significantly higher than the activity of the full length recombinant enzyme | Arabidopsis thaliana |
6.3.3.2 | additional information | - |
activity of recombinant enzyme lacking the mitochondrial targeting sequence is significantly higher than the activity of the full length recombinant enzyme | Solanum lycopersicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.3.2 | ATP + 5-formyltetrahydrofolate | - |
Arabidopsis thaliana | ADP + phosphate + 5,10-methylenetetrahydrofolate | - |
ir | |
6.3.3.2 | ATP + 5-formyltetrahydrofolate | - |
Pisum sativum | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? | |
6.3.3.2 | ATP + 5-formyltetrahydrofolate | - |
Arabidopsis thaliana | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
ir | |
6.3.3.2 | ATP + 5-formyltetrahydrofolate | - |
Solanum lycopersicum | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
ir | |
6.3.3.2 | ATP + 5-formyltetrahydrofolate-pentaglutamate | preferred substrate to the monoglutamyl form | Arabidopsis thaliana | ADP + phosphate + 5,10-methylenetetrahydrofolate-pentaglutamate | - |
ir | |
6.3.3.2 | additional information | enzyme is specific for the 6S-isomer | Arabidopsis thaliana | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.3.2 | monomer | 1 * 26500, recombinant truncated enzyme, amino acid sequence determination | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.3.2 | 5-FCL | - |
Pisum sativum |
6.3.3.2 | 5-FCL | - |
Arabidopsis thaliana |
6.3.3.2 | 5-FCL | - |
Solanum lycopersicum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.3.2 | 5.3 | - |
5-formyltetrahydrofolate | truncated recombinant enzyme, pH 6.0, 23°C | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.3.2 | 8.5 | - |
bicine buffer | Arabidopsis thaliana |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.3.3.2 | 5.5 | 9 | linear increase of activity to maximum at pH 8.5 | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.3.2 | ATP | dependent on | Pisum sativum | |
6.3.3.2 | ATP | dependent on | Arabidopsis thaliana | |
6.3.3.2 | ATP | dependent on | Solanum lycopersicum |