EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.15 | overexpression of wild-type and C-terminally truncated enzymes as His-tagged proteins in Escherichia coli | Methanocaldococcus jannaschii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.15 | additional information | the C-terminally truncated enzyme is 3fold less active with L-cysteine and 10fold less active with L-alanine compared too the wild-type enzyme | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.15 | cysteamine | inhibition of Cys-tRNAPro formation | Methanocaldococcus jannaschii | |
6.1.1.15 | D-cysteine-DL-homocysteine | inhibition of Cys-tRNAPro formation | Methanocaldococcus jannaschii | |
6.1.1.15 | L-cysteine ethyl ester | inhibition of Cys-tRNAPro formation | Methanocaldococcus jannaschii | |
6.1.1.15 | L-cysteine methyl ester | inhibition of Cys-tRNAPro formation | Methanocaldococcus jannaschii | |
6.1.1.15 | additional information | no inhibition of Cys-tRNAPro formation by L-sulfinic acid, L-cysteic acid, S-methyl L-cysteine, and homocysteine thiolactone | Methanocaldococcus jannaschii | |
6.1.1.15 | N-acetyl-L-cysteine | inhibition of Cys-tRNAPro formation | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.15 | ATP + L-proline + tRNAPro | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.15 | Methanocaldococcus jannaschii | - |
gene proS | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.15 | recombinant His-tagged wild-type and C-terminally truncated enzymes from overexpressing Escherichia coli | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.15 | ATP + L-alanine + tRNAPro | misacetylation of tRNAPro, no editing | Methanocaldococcus jannaschii | AMP + diphosphate + L-alanyl-tRNAPro | - |
? | |
6.1.1.15 | ATP + L-cysteine + tRNAPro | misacetylation of tRNAPro, no editing | Methanocaldococcus jannaschii | AMP + diphosphate + L-cysteinyl-tRNAPro | - |
? | |
6.1.1.15 | ATP + L-proline + tRNAPro | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
6.1.1.15 | additional information | enzyme does not perform deacetylation reaction of mischarged Cys-tRNAPro and Ala-tRNAPro, the enzyme also performs the ATP-diphosphate exchange reaction, no charging of tRNACys with L-cysteine | Methanocaldococcus jannaschii | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.15 | Global RNA synthesis factor | - |
Methanocaldococcus jannaschii |
6.1.1.15 | Pro-tRNA synthetase | - |
Methanocaldococcus jannaschii |
6.1.1.15 | Proline translase | - |
Methanocaldococcus jannaschii |
6.1.1.15 | Proline--tRNA ligase | - |
Methanocaldococcus jannaschii |
6.1.1.15 | Prolyl RNA synthetase | - |
Methanocaldococcus jannaschii |
6.1.1.15 | Prolyl-transfer ribonucleate synthetase | - |
Methanocaldococcus jannaschii |
6.1.1.15 | Prolyl-transfer ribonucleic acid synthetase | - |
Methanocaldococcus jannaschii |
6.1.1.15 | Prolyl-transfer RNA synthetase | - |
Methanocaldococcus jannaschii |
6.1.1.15 | Prolyl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
6.1.1.15 | ProRS | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.15 | 50 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.15 | 7 | - |
aminoacylation assay at | Methanocaldococcus jannaschii |
6.1.1.15 | 7.2 | - |
ATP-diphosphate exchange assay at | Methanocaldococcus jannaschii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.15 | ATP | - |
Methanocaldococcus jannaschii |