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Literature summary extracted from

  • Holmes, W.B.; Appling, D.R.
    Cloning and characterization of methenyltetrahydrofolate synthetase from Saccharomyces cerevisiae (2002), J. Biol. Chem., 277, 20205-20213.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.3.2 gene FAU1, orf YER183c, functional expression as His-tagged enzyme in Escherichia coliBL21(DE3) Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
6.3.3.2 additional information disruption mutants do not evoke an altered phenotype Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.3.2 additional information enzyme is inhibited by salt, therefore MgATP2- is used as cofactor instead of MgCl2 and NaATP- Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.2 0.033
-
 5-formyltetrahydrofolate recombinant enzyme, pH 7.0, 30°C Saccharomyces cerevisiae
6.3.3.2 0.043
-
 ATP recombinant enzyme, pH 7.0, 30°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.3.2 ATP + 5-formyltetrahydrofolate Saccharomyces cerevisiae
-
 ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.2 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.2 recombinant His-tagged enzyme from Escherichia coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.3.2 additional information
-
 activity in disruption mutant strains Saccharomyces cerevisiae
6.3.3.2 7
-
 purified recombinant enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Saccharomyces cerevisiae ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ir
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Saccharomyces cerevisiae ADP + phosphate + 5,10-methenyltetrahydrofolate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.3.2 Methenyltetrahydrofolate synthetase
-
 Saccharomyces cerevisiae
6.3.3.2 MTHFS
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.3.2 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.3.2 7
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.3.2 MgATP2- enzyme is inhibited by salt, therefore MgATP2- is used as cofactor instead of MgCl2 and NaATP- Saccharomyces cerevisiae