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Literature summary extracted from

  • Ahuja, A.; Purcarea, C.; Guy, H.I.; Evans, D.R.
    A novel carbamoyl-phosphate synthetase from Aquifex aeolicus (2001), J. Biol. Chem., 276, 45694-45703.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.5 ornithine
-
 Aquifex aeolicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.5 expression in Escherichia coli Aquifex aeolicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 UMP
-
 Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.5 additional information
-
 additional information
-
 Aquifex aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.5 45000
-
 CPS.A-subunit, CPS.B-subunit and glutaminase-subunit, 1* 64000 Da, 1 * 63000 Da and 1 * 45000 Da, but only the CPS.A-CPS.B-dimer has catalytic activity, SDS-PAGE Aquifex aeolicus
6.3.5.5 63000
-
 CPS.A-subunit, CPS.B-subunit and glutaminase-subunit, 1* 64000 Da, 1 * 63000 Da and 1 * 45000 Da, but only the CPS.A-CPS.B-dimer has catalytic activity, SDS-PAGE Aquifex aeolicus
6.3.5.5 171000
-
-
 Aquifex aeolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3- Aquifex aeolicus the product carbamoyl phosphate is the initial intermediate in the biosynthesis of both pyrimidine and arginine 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Aquifex aeolicus
-
-
-
6.3.5.5 Bacillus subtilis
-
-
-
6.3.5.5 Cricetinae
-
-
-
6.3.5.5 Escherichia coli
-
-
-
6.3.5.5 Methanocaldococcus jannaschii
-
-
-
6.3.5.5 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.5 recombinant enzyme Aquifex aeolicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.5 0.041
-
 recombinant enzyme Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Bacillus subtilis 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Pseudomonas aeruginosa 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Methanocaldococcus jannaschii 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Cricetinae 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Aquifex aeolicus 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + L-Gln + HCO3- the product carbamoyl phosphate is the initial intermediate in the biosynthesis of both pyrimidine and arginine Aquifex aeolicus 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
 Aquifex aeolicus 2 ADP + phosphate + carbamoyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.5 heterotrimer CPS.A-subunit, CPS.B-subunit and glutaminase-subunit, 1* 64000 Da, 1 * 63000 Da and 1 * 45000 Da, but only the CPS.A-CPS.B-dimer has catalytic activity, SDS-PAGE Aquifex aeolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.5.5 70 80 CPS.A-CPS.B complex Aquifex aeolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.5.5 25 80 The activity of the CPS.A-CPS.B complex remains unchanged between 25-60 °C and then increases 54% at 70°C. The loss of catalytic activity above 80°C is indicated of thermal denaturation. The large increase in thermal stability at temperatures prior to thermal denaturation is not observed in the CPS.A-CPS.B-glutaminase-subunit complex. Aquifex aeolicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.5 additional information
-
 additional information
-
 Aquifex aeolicus