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Literature summary extracted from

  • Beuning, P.J.; Musier-Forsyth, K.
    Species-specific differences in amino acid editing by class II prolyl-tRNA synthetase (2001), J. Biol. Chem., 276, 30779-30785.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.1.1.15 pharmacology enzyme is a target for design of antibiotics targeting the editing active site since eukaryotic enzyme types are not able to edit misactivated alanine on tRNAPro Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.15 0.012
-
L-proline 60°C, pH not specified in the publication, ATP-diphosphate exchange assay Methanocaldococcus jannaschii
6.1.1.15 0.18
-
L-proline 37°C Homo sapiens
6.1.1.15 0.25
-
L-proline 37°C Escherichia coli
6.1.1.15 79
-
L-alanine 37°C Homo sapiens
6.1.1.15 140
-
L-alanine 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro Escherichia coli
-
AMP + diphosphate + L-prolyl-tRNAPro
-
?
6.1.1.15 ATP + L-proline + tRNAPro Homo sapiens
-
AMP + diphosphate + L-prolyl-tRNAPro
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Escherichia coli
-
-
-
6.1.1.15 Homo sapiens
-
-
-
6.1.1.15 Methanocaldococcus jannaschii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-alanine + tRNAPro very low activity Homo sapiens AMP + diphosphate + L-alanyl-tRNAPro
-
?
6.1.1.15 ATP + L-alanine + tRNAPro enzyme possesses both pre- and post-transfer hydrolytic editing activity to prevent from misincorporation of alanine into proteins Escherichia coli AMP + diphosphate + L-alanyl-tRNAPro
-
?
6.1.1.15 ATP + L-proline + tRNAPro
-
Escherichia coli AMP + diphosphate + L-prolyl-tRNAPro
-
?
6.1.1.15 ATP + L-proline + tRNAPro
-
Escherichia coli AMP + diphosphate + L-prolyl-tRNAPro
-
ir
6.1.1.15 ATP + L-proline + tRNAPro
-
Homo sapiens AMP + diphosphate + L-prolyl-tRNAPro
-
?
6.1.1.15 ATP + L-proline + tRNAPro the enzyme mischarges tRNAPro with alanine at a rate that is 6800fold reduced relative to charging with proline. The enzyme is able to hydrolyze misactivated alanine via both pretransfer and post-transfer editing pathways. Mischarging of a tRNAPro transcript with cysteine is also detected. The enzyme stimulates ATP hydrolysis activity with the noncognate amino acid alanine but not in the presence of proline Methanocaldococcus jannaschii AMP + diphosphate + L-prolyl-tRNAPro
-
?
6.1.1.15 additional information no activity with L-cysteine, the enzyme also performs the ATP-diphosphate exchange reaction, enzyme possesses no editing activity against L-alanine and to prevent from misincorporation of alanine into proteins Homo sapiens ?
-
?
6.1.1.15 additional information the enzyme also performs the ATP-diphosphate exchange reaction, no activity with L-cysteine Escherichia coli ?
-
?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.15 Global RNA synthesis factor
-
Escherichia coli
6.1.1.15 Global RNA synthesis factor
-
Homo sapiens
6.1.1.15 Pro-tRNA synthetase
-
Escherichia coli
6.1.1.15 Pro-tRNA synthetase
-
Homo sapiens
6.1.1.15 Proline translase
-
Escherichia coli
6.1.1.15 Proline translase
-
Homo sapiens
6.1.1.15 Proline--tRNA ligase
-
Escherichia coli
6.1.1.15 Proline--tRNA ligase
-
Homo sapiens
6.1.1.15 Prolyl RNA synthetase
-
Escherichia coli
6.1.1.15 Prolyl RNA synthetase
-
Homo sapiens
6.1.1.15 Prolyl-transfer ribonucleate synthetase
-
Escherichia coli
6.1.1.15 Prolyl-transfer ribonucleate synthetase
-
Homo sapiens
6.1.1.15 Prolyl-transfer ribonucleic acid synthetase
-
Escherichia coli
6.1.1.15 Prolyl-transfer ribonucleic acid synthetase
-
Homo sapiens
6.1.1.15 Prolyl-transfer RNA synthetase
-
Escherichia coli
6.1.1.15 Prolyl-transfer RNA synthetase
-
Homo sapiens
6.1.1.15 Prolyl-tRNA synthetase
-
Escherichia coli
6.1.1.15 Prolyl-tRNA synthetase
-
Homo sapiens
6.1.1.15 ProRS
-
Escherichia coli
6.1.1.15 ProRS
-
Homo sapiens
6.1.1.15 ProRS
-
Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.15 37
-
assay at Escherichia coli
6.1.1.15 37
-
assay at Homo sapiens
6.1.1.15 60
-
assay at Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.15 0.11
-
L-alanine 37°C Homo sapiens
6.1.1.15 1.7
-
L-alanine 37°C Escherichia coli
6.1.1.15 70
-
L-proline 37°C Escherichia coli
6.1.1.15 80
-
L-proline 37°C Homo sapiens
6.1.1.15 210
-
L-proline 60°C, pH not specified in the publication, ATP-diphosphate exchange assay Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.15 ATP
-
Escherichia coli
6.1.1.15 ATP
-
Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.15 12000
-
L-proline 60°C, pH not specified in the publication, ATP-diphosphate exchange assay Methanocaldococcus jannaschii