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Literature summary extracted from
- A 33-kDa allergen from rice (Oryza sativa L. Japonica). cDNA cloning, expression, and identification as a novel glyoxalase I (2001), J. Biol. Chem., 276, 11376-11381.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.4.1.5 | cloning of cDNA, expression in Escherichia coli | Oryza sativa Japonica Group |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.5 | 7.6 | - |
glutathione-methylglyoxal hemithioacetal | 25°C, pH 7.0, native Glb33 | Oryza sativa Japonica Group |  |
| 4.4.1.5 | 11 | - |
glutathione-methylglyoxal hemithioacetal | 25°C, pH 7.0, recombinant Glb33 | Oryza sativa Japonica Group | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.5 | 33000 | - |
x * 33000, SDS-PAGE, native and recombinant Glb33 | Oryza sativa Japonica Group |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.5 | glutathione-methylglyoxal hemithioacetal | Oryza sativa Japonica Group | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | (R)-S-lactoylglutathione | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.5 | Oryza sativa Japonica Group | Q948T6 | L. Japonica | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.4.1.5 | native and recombinant glyoxalase I | Oryza sativa Japonica Group |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.5 | glutathione-methylglyoxal hemithioacetal | glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione | Oryza sativa Japonica Group | (R)-S-lactoylglutathione | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.4.1.5 | ? | x * 33000, SDS-PAGE, native and recombinant Glb33 | Oryza sativa Japonica Group |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.4.1.5 | Glb33 | - |
Oryza sativa Japonica Group |
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