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Literature summary extracted from
- Characterization and subcellular localization of murine and human magnesium-dependent neutral sphingomyelinase (2000), J. Biol. Chem., 275, 5710-5717.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | arachidonic acid | approx. 3fold activation at 0.5 mM | Mus musculus |  |
| 3.1.4.12 | arachidonic acid | approx. 3fold activation at 0.5 mM | Homo sapiens | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | expression in Escherichia coli and HEK293 kidney cells | Mus musculus |
| 3.1.4.12 | expression in Escherichia coli and HEK293 kidney cells | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | E49Q | complete loss of hnSMase activity | Homo sapiens |
| 3.1.4.12 | H272N | complete loss of hnSMase activity | Homo sapiens |
| 3.1.4.12 | N180H | complete loss of hnSMase activity | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | Mn2+ | above 0.5 mM | Homo sapiens | |
| 3.1.4.12 | Mn2+ | above 0.5 mM | Mus musculus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.12 | smooth endoplasmic reticulum | - |
Mus musculus | 5790 | - |
| 3.1.4.12 | smooth endoplasmic reticulum | - |
Homo sapiens | 5790 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | Mg2+ | required for activity, maximal activation at 2-5 mM | Mus musculus | |
| 3.1.4.12 | Mg2+ | required for activity, maximal activation at 2-5 mM | Homo sapiens | |
| 3.1.4.12 | Mn2+ | can replace Mg2+, maximal activity at 0.5 mM, inhibition above | Mus musculus | |
| 3.1.4.12 | Mn2+ | can replace Mg2+, maximal activity at 0.5 mM, inhibition above | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 42000 | - |
x * 42000, SDS-PAGE | Mus musculus |
| 3.1.4.12 | 42000 | - |
x * 42000, SDS-PAGE | Homo sapiens |
| 3.1.4.12 | 47500 | - |
x * 47500, deduced from nucleotide sequence | Mus musculus |
| 3.1.4.12 | 47600 | - |
x * 47600, deduced from nucleotide sequence | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | sphingomyelin + H2O | Mus musculus | - |
N-acylsphingosine + choline phosphate | - |
? | |
| 3.1.4.12 | sphingomyelin + H2O | Homo sapiens | - |
N-acylsphingosine + choline phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | Homo sapiens | - |
- |
- |
| 3.1.4.12 | Mus musculus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | no glycoprotein | - |
Mus musculus |
| 3.1.4.12 | no glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | affinity purification of recombinant hnSMase | Homo sapiens |
| 3.1.4.12 | affinity purification of recombinant mnSMase | Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | brain | - |
Mus musculus | - |
| 3.1.4.12 | brain | - |
Homo sapiens | - |
| 3.1.4.12 | heart | - |
Mus musculus | - |
| 3.1.4.12 | heart | - |
Homo sapiens | - |
| 3.1.4.12 | kidney | predominant tissue | Mus musculus | - |
| 3.1.4.12 | kidney | predominant tissue | Homo sapiens | - |
| 3.1.4.12 | liver | - |
Mus musculus | - |
| 3.1.4.12 | liver | - |
Homo sapiens | - |
| 3.1.4.12 | lung | - |
Mus musculus | - |
| 3.1.4.12 | lung | - |
Homo sapiens | - |
| 3.1.4.12 | muscle | - |
Mus musculus | - |
| 3.1.4.12 | muscle | - |
Homo sapiens | - |
| 3.1.4.12 | spleen | - |
Mus musculus | - |
| 3.1.4.12 | spleen | - |
Homo sapiens | - |
| 3.1.4.12 | testis | - |
Mus musculus | - |
| 3.1.4.12 | testis | - |
Homo sapiens | - |
| 3.1.4.12 | thymus | - |
Mus musculus | - |
| 3.1.4.12 | thymus | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | sphingomyelin + H2O | - |
Mus musculus | N-acylsphingosine + choline phosphate | - |
? | |
| 3.1.4.12 | sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | ? | x * 42000, SDS-PAGE | Mus musculus |
| 3.1.4.12 | ? | x * 42000, SDS-PAGE | Homo sapiens |
| 3.1.4.12 | ? | x * 47500, deduced from nucleotide sequence | Mus musculus |
| 3.1.4.12 | ? | x * 47600, deduced from nucleotide sequence | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | hnSMase | - |
Homo sapiens |
| 3.1.4.12 | magnesium-dependent neutral sphingomyelinase | - |
Mus musculus |
| 3.1.4.12 | magnesium-dependent neutral sphingomyelinase | - |
Homo sapiens |
| 3.1.4.12 | mnSMase | - |
Mus musculus |
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