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Literature summary extracted from

  • Boyd, A.E.; Marnett, A.B.; Wong, L.; Taylor, P.
    Probing the active center gorge of acetylcholinesterase by fluorophores linked to substituted cysteines (2000), J. Biol. Chem., 275, 22401-22408.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.7
-
 Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.7 A262C mutant retains hydrolytic activity Mus musculus
3.1.1.7 H287C mutant retains hydrolytic activity Mus musculus
3.1.1.7 Y124C mutant retains hydrolytic activity Mus musculus
3.1.1.7 Y124C-acrylodan mutant retains hydrolytic activity Mus musculus
3.1.1.7 Y124C-benzyl mutant retains hydrolytic activity Mus musculus
3.1.1.7 Y124C-N(CH3)3+ mutant with increased Km Mus musculus
3.1.1.7 Y124C-NH3+ mutant with increased Km Mus musculus
3.1.1.7 Y124C-SO3- mutant retains hydrolytic activity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 fasciculin inhibits catalysis peripherally by sealing the mouth of the active center gorge Mus musculus
3.1.1.7 trifluoroacetophenone inhibits Y124C mutant slower than the wild-type enzyme Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.7 0.046
-
 Acetylcholine wild-type Mus musculus
3.1.1.7 0.058
-
 Acetylcholine H287C mutant Mus musculus
3.1.1.7 0.058
-
 Acetylcholine Y124C-SO3- mutant Mus musculus
3.1.1.7 0.059
-
 Acetylcholine A262C mutant Mus musculus
3.1.1.7 0.059
-
 Acetylcholine Y124C-benzyl mutant Mus musculus
3.1.1.7 0.065
-
 Acetylcholine Y124C mutant Mus musculus
3.1.1.7 0.28
-
 Acetylcholine Y124C-acrylodan mutant Mus musculus
3.1.1.7 1.13
-
 Acetylcholine Y124C-N(Ch3)3+ mutant Mus musculus
3.1.1.7 2.36
-
 Acetylcholine Y124C-NH3+ mutant Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.7 acetylcholine + H2O Mus musculus
-
 choline + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Mus musculus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 acetylcholine + H2O
-
 Mus musculus choline + acetate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.7 95
-
 Acetylcholine Y124C-acrylodan mutant Mus musculus
3.1.1.7 1330
-
 Acetylcholine Y124C-benzyl mutant Mus musculus
3.1.1.7 1333
-
 Acetylcholine Y124C-benzyl mutant Mus musculus
3.1.1.7 1850
-
 Acetylcholine Y124C-N(Ch3)3+ mutant Mus musculus
3.1.1.7 2130
-
 Acetylcholine Y124C-SO3- mutant Mus musculus
3.1.1.7 2133
-
 Acetylcholine Y124C-SO3- mutant Mus musculus
3.1.1.7 2330
-
 Acetylcholine wild-type Mus musculus
3.1.1.7 2333
-
 Acetylcholine wild-type Mus musculus
3.1.1.7 2367
-
 Acetylcholine Y124C mutant Mus musculus
3.1.1.7 2370
-
 Acetylcholine Y124C mutant Mus musculus
3.1.1.7 2730
-
 Acetylcholine A262C mutant Mus musculus
3.1.1.7 2733
-
 Acetylcholine A262C mutant Mus musculus
3.1.1.7 3000
-
 Acetylcholine H287C mutant Mus musculus
3.1.1.7 4350
-
 Acetylcholine Y124C-NH3+ mutant Mus musculus