EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.20 | D56A | site-directed mutagenesis, mutation in the alpha-subunit, replacement of the residue, which builds a salt bridge to the alpha-subunit, has deleterious effects, which can be repaired by increased temperature in combination with CsCl or NaCl plus alpha-glycerol 3-phosphate, an alpha-ligand, alteration of temperature dependence of the enzyme complex | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.20 | K167T | site-directed mutagenesis, mutation in the beta-subunit, replacement of the residue, which builds a salt bridge to the alpha-subunit, has deleterious effects, which can be repaired by increased temperature in combination with CsCl or NaCl plus alpha-glycerol 3-phosphate, an alpha-ligand, alteration of temperature dependence of the enzyme complex | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.20 | DL-glycerol 3-phosphate | wild-type and mutant enzymes, in presence of monovalent cations | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.2.1.20 | Na+ | mutants D56A and K167T | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.20 | Cs+ | activation of mutants D56A and K167T | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.2.1.20 | additional information | monovalent cations are involved in the thermal stability and activity equilibrium of the enzyme complex, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.20 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.2.1.20 | L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), reaction mechanism, formation of intermediates in the alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.20 | 70 | - |
mutant K167T, in presence of Na+ | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.20 | 75 | - |
mutant D56A, in presence of Na+ | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.20 | 1310 | - |
wild-type enzyme, in presence of Na+ | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.20 | 1400 | - |
wild-type enzyme, in presence of Cs+ | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.20 | 1650 | - |
mutant K167T, in presence of Cs+ | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.20 | 2200 | - |
mutant D56A, in presence of Cs+ | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.20 | 1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | D-glyceraldehyde 3-phosphate + indole | - |
? | |
4.2.1.20 | L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.20 | More | structure and conformation regulating the activity and allosteric communication in the enzyme complex, modeling of the high activity closed form and the low activity open form | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.20 | tetramer | alpha2beta2 complex | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.20 | 37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.20 | additional information | - |
temperature-dependent changes in equilibrium distribution of enzyme-substrate intermediates and in primary kinetic isotope effect, D56 and K167 are involved | Salmonella enterica subsp. enterica serovar Typhimurium |