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Literature summary extracted from
- Lys13 plays a crucial role in the functional adaptation of the thermophilic triose-phosphate isomerase from Bacillus stearothermophilus to high temperatures (1999), J. Biol. Chem., 274, 19181-19187.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | - |
Geobacillus stearothermophilus |
| 5.3.1.1 | - |
Thermotoga maritima |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | hanging drop vapor diffusion method | Geobacillus stearothermophilus |
| 5.3.1.1 | hanging drop vapor diffusion method | Thermotoga maritima |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.1.1 | H12N | mutant enzyme with decreased thermal stability compared to wild-type enzyme. Half-life of 11.5 min at 64°C compared to 68°C for the wild-type enzyme | Geobacillus stearothermophilus |
| 5.3.1.1 | H12N/K13G | mutant enzyme shows increases thermal stability compared to wild-type enzyme. Half-life of 11.5 min at 96°C compared to 94°C of the wild-type enzyme | Thermotoga maritima |
| 5.3.1.1 | H12N/K13G | mutant enzyme with decreased thermal stability compared to wild-type enzyme. Half-life of 11.5 min at 37°C compared to 68°C for the wild-type enzyme | Geobacillus stearothermophilus |
| 5.3.1.1 | H13G | mutant enzyme with decreased thermal stability compared to wild-type enzyme. Half-life of 11.5 min at 51°C compared to 68°C for the wild-type enzyme | Geobacillus stearothermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.1 | Geobacillus stearothermophilus | - |
- |
- |
| 5.3.1.1 | Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | wild-type and mutant enzyme H12N/K13G | Thermotoga maritima |
| 5.3.1.1 | wild-type and mutant enzymes H12N, H13G and H12N/K13G | Geobacillus stearothermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | additional information | - |
Lys13 plays a crucial role in the functional adaptation of the thermophilic enzyme to high temperatures | Geobacillus stearothermophilus |
| 5.3.1.1 | 37 | - |
half-life of mutant enzyme H12N/K13G is 11.5 min | Geobacillus stearothermophilus |
| 5.3.1.1 | 51 | - |
half-life of mutant enzyme H13G is 11.5 min | Geobacillus stearothermophilus |
| 5.3.1.1 | 64 | - |
half-life of mutant enzyme H12N is 11.5 min | Geobacillus stearothermophilus |
| 5.3.1.1 | 68 | - |
half-life of wild-type recombinant enzyme is 11.5 min | Geobacillus stearothermophilus |
| 5.3.1.1 | 94 | - |
half-life of wild-type recombinant enzyme is 11.5 min | Thermotoga maritima |
| 5.3.1.1 | 96 | - |
half-life of mutant enzyme H12N/K13G is 11.5 min | Thermotoga maritima |
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