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Literature summary extracted from

  • Barkley, S.J.; Cornish, R.M.; Poulter, C.D.
    Identification of an archaeal type II isopentenyl diphosphate isomerase in Methanothermobacter thermautotrophicus (2004), J. Bacteriol., 186, 1811-1817.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.3.2 FMN required as cofactor. The enzyme requires the combined presence of FMN, NADPH and Mg2+ Methanothermobacter thermautotrophicus
5.3.3.2 NADPH required as cofactor. The enzyme requires the combined presence of FMN, NADPH and Mg2+ Methanothermobacter thermautotrophicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.2 expression in Escherichia coli, His6-tagged type II enzyme Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.2 0.064
-
 isopentenyl diphosphate
-
 Methanothermobacter thermautotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.3.2 Mg2+ required. The enzyme requires the combined presence of FMN, NADPH and Mg2+ Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.2 48000
-
 4 * 48000, SDS-PAGE Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.2 isopentenyl diphosphate Methanothermobacter thermautotrophicus the enzyme catalyzes the interconversion of the fundamental five carbon homoallylic and allylic diphosphate building blocks required for biosynthesis of isoprenoid compounds dimethylallyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.2 Methanothermobacter thermautotrophicus
-
 type II isopentenyl diphosphate isomerase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.2 recombinant enzyme Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.2 isopentenyl diphosphate
-
 Methanothermobacter thermautotrophicus dimethylallyl diphosphate
-
 ?
5.3.3.2 isopentenyl diphosphate the enzyme catalyzes the interconversion of the fundamental five carbon homoallylic and allylic diphosphate building blocks required for biosynthesis of isoprenoid compounds Methanothermobacter thermautotrophicus dimethylallyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.3.2 tetramer 4 * 48000, SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.3.2 70
-
-
 Methanothermobacter thermautotrophicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.3.2 1.6
-
 isopentenyl diphosphate
-
 Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.3.2 6.5
-
-
 Methanothermobacter thermautotrophicus