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Literature summary extracted from

  • Sueyoshi, N.; Kita, K.; Okino, N.; Sakaguchi, K.; Nakamura, T.; Ito, M.
    Molecular cloning and expression of Mn2+-dependent sphingomyelinase/hemolysin of an aquatic bacterium, Pseudomonas sp. strain TK4 (2002), J. Bacteriol., 184, 540-546.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 expression in Escherichia coli Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.12 additional information C-Terminal deletion mutants lose sphingomyelinase activity for hydrolysis of intact erythrocytes Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 Ca2+ strong inhibition of hemolysis at 10 mM Pseudomonas sp.
3.1.4.12 Cu2+ 5 mM, complete inhibition Pseudomonas sp.
3.1.4.12 EDTA 5 mM, complete inhibition, subsequent addition of Mn2+, Mg2+ or Ca2+ restores activity to 300% of that before EDTA treatment Pseudomonas sp.
3.1.4.12 Hg2+ 5 mM, complete inhibition Pseudomonas sp.
3.1.4.12 Ni2+ 5 mM, complete inhibition Pseudomonas sp.
3.1.4.12 Zn2+ 5 mM, complete inhibition Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Ca2+ activation of hemolysis at 2 mM, strong inhibition at 10 mM Pseudomonas sp.
3.1.4.12 Mg2+ 5 mM, activation Pseudomonas sp.
3.1.4.12 Mn2+ 5 mM, activation Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 58110
-
 x * 58110, deduced from nucleotide sequence Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Pseudomonas sp. SMase C causes hemolysis and thus is suspected to be a potential virulence factor N-acylsphingosine + choline phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 HighTrap Phenyl-sepharose, HiTrap Q, recombinant SMase Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.12 33
-
 recombinant SMase C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 4-nitrobenz-2-oxa-1,3-diazole-sphingosylphosphorylcholine + H2O
-
 Pseudomonas sp. 4-nitrobenz-2-oxa-1,3-diazole-sphingosine + choline phosphate
-
 ?
3.1.4.12 lysophosphatidylcholine + H2O 8% of activity with sphingomyelin Pseudomonas sp. acylglycerol + choline phosphate
-
 ?
3.1.4.12 N-dodecanoyl-4-nitrobenz-2-oxa-1,3-diazole-sphingomyelin + H2O
-
 Pseudomonas sp. 4-nitrobenz-2-oxa-1,3-diazole-N-dodecanoylsphingosine + choline phosphate
-
 ?
3.1.4.12 N-hexanoyl-4-nitrobenz-2-oxa-1,3-diazole-sphingomyelin + H2O
-
 Pseudomonas sp. 4-nitrobenz-2-oxa-1,3-diazole-N-hexanoylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Pseudomonas sp. N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O SMase C causes hemolysis and thus is suspected to be a potential virulence factor Pseudomonas sp. N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingosylphosphorylcholine + H2O 25% of activity with sphingomyelin Pseudomonas sp. sphingosine + choline phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.12 ? x * 58110, deduced from nucleotide sequence Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 SMase C
-
 Pseudomonas sp.
3.1.4.12 sphingomyelinase/hemolysin
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 8
-
-
 Pseudomonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.4.12 Pseudomonas sp. deduced from nucleotide sequence
-
 6.33