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Literature summary extracted from

  • Kim, A.D.; Graham, D.E.; Seeholzer, S.H.; Markham, G.D.
    S-Adenosylmethionine decarboxylase from the archaeon Methanococcus jannaschii: identification of a novel family of pyruvoyl enzymes (2000), J. Bacteriol., 182, 6667-6672.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.50
-
 Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.50 additional information not inhibitory are: MgCl2, CaCl2, EDTA, putrescine at 10 mM each, or KCl at 50 mM Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.50 0.12
-
 S-adenosyl-L-methionine pH 7.4, 25°C Methanocaldococcus jannaschii
4.1.1.50 0.6
-
 S-adenosyl-L-methionine pH 7.4, 70°C Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.50 additional information no significant levels of metals Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.50 6000
-
 2 * 6000, alpha, + 2 * 8000, beta, SDS-PAGE, 2 * 6793 * 2 * 6990, MALDI-TOF Methanocaldococcus jannaschii
4.1.1.50 6793
-
 2 * 6000, alpha, + 2 * 8000, beta, SDS-PAGE, 2 * 6793 * 2 * 6990, MALDI-TOF Methanocaldococcus jannaschii
4.1.1.50 6990
-
 2 * 6000, alpha, + 2 * 8000, beta, SDS-PAGE, 2 * 6793 * 2 * 6990, MALDI-TOF Methanocaldococcus jannaschii
4.1.1.50 8000
-
 2 * 6000, alpha, + 2 * 8000, beta, SDS-PAGE, 2 * 6793 * 2 * 6990, MALDI-TOF Methanocaldococcus jannaschii
4.1.1.50 30000
-
 PAGE Methanocaldococcus jannaschii
4.1.1.50 35000
-
 gel filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.50 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.50 after expression in Escherichia coli Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.50 S-adenosyl-L-methionine
-
 Methanocaldococcus jannaschii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.50 heterotetramer 2 * 6000, alpha, + 2 * 8000, beta, SDS-PAGE, 2 * 6793 * 2 * 6990, MALDI-TOF Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.50 70
-
 3 h, full stability Methanocaldococcus jannaschii