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Literature summary extracted from

  • Tanner, A.; Hopper, D.J.
    Conversion of 4-hydroxyacetophenone into 4-phenyl acetate by a flavin adenine dinucleotide-containing Baeyer-Villiger-type monooxygenase (2000), J. Bacteriol., 182, 6565-6569.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.84 0.0175
-
NADPH pH 8, 30°C, 4-hydroxyacetophenone as substrate Pseudomonas putida
1.14.13.84 0.023
-
4-hydroxypropiophenone pH 8, 30°C Pseudomonas putida
1.14.13.84 0.047
-
4-hydroxyacetophenone pH 8, 30°C Pseudomonas putida
1.14.13.84 0.384
-
acetophenone pH 8, 30°C Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.84 70000
-
gel filtration Pseudomonas putida
1.14.13.84 71000
-
1 * 71000, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.84 4-hydroxyacetophenone + NADPH + H+ + O2 Pseudomonas putida involved in the Baeyer-Villiger oxygenation of 4-hydroxyacetophenone, catalyzes one of the steps in the catabolism of 4-ethylphenol, inducible enzyme 4-hydroxyphenyl acetate + NADP+ + H2O
-
?
1.14.13.84 4-hydroxyacetophenone + NADPH + H+ + O2 Pseudomonas putida JD1 involved in the Baeyer-Villiger oxygenation of 4-hydroxyacetophenone, catalyzes one of the steps in the catabolism of 4-ethylphenol, inducible enzyme 4-hydroxyphenyl acetate + NADP+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.84 Pseudomonas putida
-
-
-
1.14.13.84 Pseudomonas putida JD1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.84 98fold Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.84 (4-hydroxyphenyl)ethan-1-one + NADPH + H+ + O2 = 4-hydroxyphenyl acetate + NADP+ + H2O contains FAD, the enzyme from Pseudomonas fluorescens ACB catalyses the conversion of a wide range of acetophenone derivatives, highest activity occurs with compounds bearing an electron-donating substituent at the para position of the aromatic ring, in the absence of substrate, the enzyme can act as an NADPH oxidase (EC 1.6.3.1) Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.84 8.1
-
pH 8, 30°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.84 4-hydroxyacetophenone + NADPH + H+ + O2 consumption of one molecule of oxygen and oxidation of one molecule of NADPH per substrate molecule, specific for NADPH as electron donor, requirement for O2 Pseudomonas putida 4-hydroxyphenyl acetate + NADP+ + H2O
-
?
1.14.13.84 4-hydroxyacetophenone + NADPH + H+ + O2 involved in the Baeyer-Villiger oxygenation of 4-hydroxyacetophenone, catalyzes one of the steps in the catabolism of 4-ethylphenol, inducible enzyme Pseudomonas putida 4-hydroxyphenyl acetate + NADP+ + H2O
-
?
1.14.13.84 4-hydroxyacetophenone + NADPH + H+ + O2 consumption of one molecule of oxygen and oxidation of one molecule of NADPH per substrate molecule, specific for NADPH as electron donor, requirement for O2 Pseudomonas putida JD1 4-hydroxyphenyl acetate + NADP+ + H2O
-
?
1.14.13.84 4-hydroxyacetophenone + NADPH + H+ + O2 involved in the Baeyer-Villiger oxygenation of 4-hydroxyacetophenone, catalyzes one of the steps in the catabolism of 4-ethylphenol, inducible enzyme Pseudomonas putida JD1 4-hydroxyphenyl acetate + NADP+ + H2O
-
?
1.14.13.84 4-hydroxypropiophenone + NADPH + H+ + O2 specific for NADPH as electron donor, requirement for O2 Pseudomonas putida 4-hydroxyphenyl propionate + NADP+ + H2O
-
?
1.14.13.84 4-hydroxypropiophenone + NADPH + H+ + O2 specific for NADPH as electron donor, requirement for O2 Pseudomonas putida JD1 4-hydroxyphenyl propionate + NADP+ + H2O
-
?
1.14.13.84 acetophenone + NADPH + H+ + O2 specific for NADPH as electron donor, requirement for O2 Pseudomonas putida phenyl acetate + NADP+ + H2O
-
?
1.14.13.84 acetophenone + NADPH + H+ + O2 specific for NADPH as electron donor, requirement for O2 Pseudomonas putida JD1 phenyl acetate + NADP+ + H2O
-
?
1.14.13.84 additional information not: benzophenone, cyclohexylacetone, NADH Pseudomonas putida ?
-
?
1.14.13.84 additional information not: benzophenone, cyclohexylacetone, NADH Pseudomonas putida JD1 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.14.13.84 monomer 1 * 71000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.13.84 arylketone monooxygenase
-
Pseudomonas putida
1.14.13.84 HAPMO
-
Pseudomonas putida
1.14.13.84 More Baeyer-Villiger-type monooxygenase Pseudomonas putida
1.14.13.84 oxygenase, 4-hydroxyacetophenone mono-
-
Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.84 30
-
assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.84 8
-
Tris-HCl buffer Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.84 FAD contains one molecule of FAD per enzyme molecule, FAD is reduced specifically by NADPH and not by NADH Pseudomonas putida
1.14.13.84 additional information not: NADH Pseudomonas putida
1.14.13.84 NADPH uses NADPH as cofactor Pseudomonas putida