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Literature summary extracted from

  • Pecina, A.; Pascual, A.; Paneque, A.
    Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: purification and characterization of the enzyme (1999), J. Bacteriol., 181, 1409-1414.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.3 additional information not affected by EDTA Azotobacter chroococcum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 gene algL, DNA and amino acid sequence determination and analysis, cloning and overexpression in Escherichia coli Azotobacter chroococcum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 additional information not affected by EDTA Azotobacter chroococcum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 K+ highly activating, activation is synergistic with Mg2+ Azotobacter chroococcum
4.2.2.3 Mg2+ slightly activating, activation is synergistic with K+ Azotobacter chroococcum
4.2.2.3 additional information not affected by Ca2+, Co2+, Mn2+, and Zn2+ Azotobacter chroococcum
4.2.2.3 Na+ slightly activating Azotobacter chroococcum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 43000
-
 1 * 43000, SDS-PAGE Azotobacter chroococcum
4.2.2.3 46000
-
 gel filtration Azotobacter chroococcum

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Azotobacter chroococcum O50660 strain ATCC 4412, gene algL
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 recombinant enzyme from Escherichia coli, 10.1fold Azotobacter chroococcum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.3 93.88
-
 purified enzyme Azotobacter chroococcum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginic acid
-
 Azotobacter chroococcum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 monomer 1 * 43000, SDS-PAGE Azotobacter chroococcum

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase
-
 Azotobacter chroococcum
4.2.2.3 AlgL
-
 Azotobacter chroococcum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 30
-
-
 Azotobacter chroococcum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7.5
-
 assay at Azotobacter chroococcum