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Literature summary extracted from

  • Wong, B.J.; Gerlt, J.A.
    Divergent function in the crotonase superfamily: an anhydride intermediate in the reaction catalyzed by 3-hydroxyisobutyryl-CoA hydrolase (2003), J. Am. Chem. Soc., 125, 12076-12077.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.4 D151N reduced activity Pseudomonas aeruginosa
3.1.2.4 E143A no detectable activity Pseudomonas aeruginosa
3.1.2.4 E143D no detectable activity Pseudomonas aeruginosa
3.1.2.4 E143Q no detectable activity Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.4 Pseudomonas aeruginosa
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.2.4 3-hydroxy-2-methylpropanoyl-CoA + H2O = CoA + 3-hydroxy-2-methylpropanoate formation of an anhydride reaction intermediate, catalytic mechanism Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.4 3-hydroxyisobutyryl-CoA + H2O formation of an anhydride reaction intermediate, Glu164 is involved Pseudomonas aeruginosa CoA + 3-hydroxyisobutyrate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.4 3-hydroxyisobutyryl-CoA hydrolase
-
Pseudomonas aeruginosa
3.1.2.4 HICH
-
Pseudomonas aeruginosa
3.1.2.4 More the enzyme belongs to the crotonase superfamily Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.4 additional information
-
additional information
-
Pseudomonas aeruginosa
3.1.2.4 1
-
3-hydroxyisobutyryl-CoA mutant D151N Pseudomonas aeruginosa
3.1.2.4 89
-
3-hydroxyisobutyryl-CoA wild-type enzyme, a deuterium isotope of the substrate is used Pseudomonas aeruginosa