Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Magboul, A.A.A.; McSweeney, P.L.H.
    Purification and properties of an acid phosphatase from Lactobacillus curvatus DPC2024 (2000), Int. Dairy J., 9, 849-855.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.2 1,10-phenanthroline 10 mM, activation to 156% of control Latilactobacillus curvatus
3.1.3.2 EDTA 10 mM, activation to 171% of control Latilactobacillus curvatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 2-mercaptoethanol 10 mM, 27% inhibition Latilactobacillus curvatus
3.1.3.2 BaCl2 10 mM, 16% inhibition Latilactobacillus curvatus
3.1.3.2 CaCl2 10 mM, 15% inhibition Latilactobacillus curvatus
3.1.3.2 Hexametaphosphate 10 mM, 44% inhibition Latilactobacillus curvatus
3.1.3.2 HgCl2 10 mM, 83% inhibition Latilactobacillus curvatus
3.1.3.2 MnCl2 10 mM, 65% inhibition Latilactobacillus curvatus
3.1.3.2 NaF 0.1 mM, 88% inhibition, complete inhibition at 1 mM Latilactobacillus curvatus
3.1.3.2 PCMB 10 mM, 35% inhibition Latilactobacillus curvatus
3.1.3.2 PMSF 10 mM, 85% inhibition Latilactobacillus curvatus
3.1.3.2 tripolyphosphate 10 mM, 54% inhibition Latilactobacillus curvatus
3.1.3.2 ZnCl2 11 mM, 87% inhibition Latilactobacillus curvatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 HgCl2 0.1 mM, activation to 130% of control Latilactobacillus curvatus
3.1.3.2 MnCl2 0.1 mM, activation to 119% of control Latilactobacillus curvatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 26000
-
 4 * 26000, SDS-PAGE Latilactobacillus curvatus
3.1.3.2 104000
-
 gel filtration Latilactobacillus curvatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Latilactobacillus curvatus
-
-
-
3.1.3.2 Latilactobacillus curvatus DPC2024
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2
-
 Latilactobacillus curvatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 1675
-
-
 Latilactobacillus curvatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O 5% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus 1-naphthol + phosphate
-
 ?
3.1.3.2 2-naphthyl phosphate + H2O 26% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus 2-naphthol + phosphate
-
 ?
3.1.3.2 2-naphthyl phosphate + H2O 26% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus DPC2024 2-naphthol + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Latilactobacillus curvatus 4-nitrophenol + phosphate
-
 ?
3.1.3.2 5'-ADP + H2O 3% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus ?
-
 ?
3.1.3.2 5'-ADP + H2O 3% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus DPC2024 ?
-
 ?
3.1.3.2 5'-AMP + H2O 13% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus adenosine + phosphate
-
 ?
3.1.3.2 5'-AMP + H2O 13% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus DPC2024 adenosine + phosphate
-
 ?
3.1.3.2 5'-ATP + H2O 18% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus ?
-
 ?
3.1.3.2 5'-ATP + H2O 18% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus DPC2024 ?
-
 ?
3.1.3.2 alpha-D-glucose 1-phosphate + H2O 9% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus alpha-D-glucose + phosphate
-
 ?
3.1.3.2 alpha-D-glucose 6-phosphate + H2O 58% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus alpha-D-glucose + phosphate
-
 ?
3.1.3.2 D-fructose 6-phosphate + H2O 87% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus D-fructose + phosphate
-
 ?
3.1.3.2 D-fructose 6-phosphate + H2O 87% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus DPC2024 D-fructose + phosphate
-
 ?
3.1.3.2 O-phospho-DL-serine + H2O 16% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus ?
-
 ?
3.1.3.2 O-phospho-DL-threonine + H2O 5% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus ?
-
 ?
3.1.3.2 O-phospho-DL-tyrosine + H2O 4% of the activity with 4-nitrophenyl phosphate Latilactobacillus curvatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 tetramer 4 * 26000, SDS-PAGE Latilactobacillus curvatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 70
-
-
 Latilactobacillus curvatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.2 40 80 40°C: about 30% of maximal activity, 80°C: about 40% of maximal activity Latilactobacillus curvatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 60
-
 pH 4.5, 60 min, about 10% loss of activity Latilactobacillus curvatus
3.1.3.2 70
-
 pH 4.5, 30 min, more than 65% of the activity is retained Latilactobacillus curvatus
3.1.3.2 75
-
 pH 4.5, 30 min, about 80% loss of activity Latilactobacillus curvatus
3.1.3.2 80
-
 pH 4.5, 10 min, about 80% loss of activity Latilactobacillus curvatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4.5
-
-
 Latilactobacillus curvatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 4 6 pH 4.0: about 60% of maximal activity, pH 6.0: about 35% of maximal activity Latilactobacillus curvatus