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Literature summary extracted from
- Characterization of a novel insect digestive DNase with a highly alkaline pH optimum (2002), Insect Biochem. Mol. Biol., 32, 255-263.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.1 | EDTA | - |
Choristoneura fumiferana |  |
| 3.1.21.1 | K+ | no inhibition at 50 mM, 50% inhibition at 200 mM | Choristoneura fumiferana | |
| 3.1.21.1 | Na+ | no inhibition at 50 mM, 50% inhibition at 200 mM | Choristoneura fumiferana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.21.1 | extracellular | - |
Choristoneura fumiferana | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.1 | Ca2+ | activates, transition state metal ions also activate, but are less effective | Choristoneura fumiferana | |
| 3.1.21.1 | Mg2+ | best activator, transition state metal ions also activate, but are less effective | Choristoneura fumiferana | |
| 3.1.21.1 | additional information | divalent alkaline metal ion requirement | Choristoneura fumiferana |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.1 | 23000 | - |
x * 23000, SDS-PAGE | Choristoneura fumiferana |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.1 | dsDNA + H2O | Choristoneura fumiferana | - |
5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
| 3.1.21.1 | additional information | Choristoneura fumiferana | enzyme is an integral component of the larval digestive system, enzyme plays an important role in the conversion of the insecticidal crystal protein from Bacillus thuringiensis to the active DNA-free toxin in the larval gut, purified enzyme acts synergistically with the toxin | ? | - |
? | |
| 3.1.21.1 | ssDNA + H2O | Choristoneura fumiferana | - |
5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.1 | Choristoneura fumiferana | - |
spruce budworm | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.21.1 | no glycoprotein | - |
Choristoneura fumiferana |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.1 | from larval gut | Choristoneura fumiferana |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products | preference for single-stranded DNA | Choristoneura fumiferana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.21.1 | gut | - |
Choristoneura fumiferana | - |
| 3.1.21.1 | larva | - |
Choristoneura fumiferana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.1 | dsDNA + H2O | - |
Choristoneura fumiferana | 5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
| 3.1.21.1 | additional information | no activity with homo-oligomers of dA, dC, dG and annealed homo-oligomers of dA/dT, no activity with RNA | Choristoneura fumiferana | ? | - |
? | |
| 3.1.21.1 | additional information | enzyme is an integral component of the larval digestive system, enzyme plays an important role in the conversion of the insecticidal crystal protein from Bacillus thuringiensis to the active DNA-free toxin in the larval gut, purified enzyme acts synergistically with the toxin | Choristoneura fumiferana | ? | - |
? | |
| 3.1.21.1 | ssDNA + H2O | - |
Choristoneura fumiferana | 5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
| 3.1.21.1 | ssDNA + H2O | preferred substrate | Choristoneura fumiferana | 5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.21.1 | ? | x * 23000, SDS-PAGE | Choristoneura fumiferana |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.1 | DNase I | - |
Choristoneura fumiferana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.1 | 10.5 | 11 | - |
Choristoneura fumiferana |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.1 | 9.5 | 12 | pH activity profile | Choristoneura fumiferana |
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Release 2023.1 (January 2023)
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