Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Myhrstad, M.C.; Carlsen, H.; Nordstrom, O.; Blomhoff, R.; Moskaug, J.O.
    Flavonoids increase the intracellular glutathione level by transactivation of the gamma-glutamylcysteine synthetase catalytical subunit promoter (2002), Free Radic. Biol. Med., 32, 386-393.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.2 apigenin nearly 2fold induction of the heavy subunit gene promotor and heavy subunit expression Homo sapiens
6.3.2.2 kaempferol 2fold induction of the heavy subunit gene promotor and heavy subunit expression Homo sapiens
6.3.2.2 additional information no increase in gene promotor activity by myricetin and sugar conjugates of quercetin, quercetin-3-glucoside and quercetin-3-rhamnoglucoside Homo sapiens
6.3.2.2 onion extract containing flavonoids, which increase the expression of both subunits of the enzyme in COS-1 cells Homo sapiens
6.3.2.2 quercetin 3fold induction of the heavy subunit gene promotor and heavy subunit expression, best at 0.05 mM, induction even of a distal part of the promotor sequence containing only 2 antioxidant-response/electrophile-response elements, i.e. ARE/EpRE Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.2 expression of a construct consisting of 3.8 kb of the enzyme's heavy subunit gene promotor in front of luciferase as well as a fragment of the 5'-flanking sequence, transient expression in COS-1 cells Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.2 cell culture
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-Glu + L-Cys
-
 Homo sapiens ADP + phosphate + gamma-L-Glu-L-Cys
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.2 dimer heavy and light subunit Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.3.2.2 gamma-glutaminylcysteine synthetase
-
 Homo sapiens
6.3.2.2 GCS
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.2 ATP
-
 Homo sapiens