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Literature summary extracted from

  • van der Oost, J.; Huynen, M.A.; Verhees, C.H.
    Molecular characterization of phosphoglycerate mutase in archaea (2002), FEMS Microbiol. Lett., 212, 111-120.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.12 expression in Escherichia coli Pyrococcus furiosus
5.4.2.12 expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.2.12 EDTA 2 mM, 20% inhibition Methanocaldococcus jannaschii
5.4.2.12 EDTA 2 mM, 20% inhibition Pyrococcus furiosus
5.4.2.12 Zn2+ 10 mM, 81% inhibition Methanocaldococcus jannaschii
5.4.2.12 Zn2+ 10 mM, 85% inhibition Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.12 0.33
-
 3-phospho-D-glycerate 50°C, pH 8.0 Methanocaldococcus jannaschii
5.4.2.12 0.49
-
 3-phospho-D-glycerate 50°C, pH 8.0 Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.12 Mg2+ 10 mM, slight activation Pyrococcus furiosus
5.4.2.12 Mg2+ 10 mM, slight activation Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.12 45200
-
 4 * 45200, deduced from nucleotide sequence Methanocaldococcus jannaschii
5.4.2.12 45300
-
 4 * 45300, deduced from nucleotide sequence Pyrococcus furiosus
5.4.2.12 183500
-
 gel filtration Pyrococcus furiosus
5.4.2.12 190600
-
 gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.12 2-phospho-D-glycerate Pyrococcus furiosus
-
 3-phospho-D-glycerate
-
 r
5.4.2.12 2-phospho-D-glycerate Methanocaldococcus jannaschii
-
 3-phospho-D-glycerate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.12 Methanocaldococcus jannaschii
-
-
-
5.4.2.12 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.12 recombinant iPGM Pyrococcus furiosus
5.4.2.12 recombinant iPGM Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.12 2-phospho-D-glycerate
-
 Pyrococcus furiosus 3-phospho-D-glycerate
-
 r
5.4.2.12 2-phospho-D-glycerate
-
 Methanocaldococcus jannaschii 3-phospho-D-glycerate
-
 r
5.4.2.12 3-phospho-D-glycerate
-
 Methanocaldococcus jannaschii 2-phospho-D-glycerate
-
 r

Subunits

EC Number Subunits Comment Organism
5.4.2.12 tetramer 4 * 45200, deduced from nucleotide sequence Methanocaldococcus jannaschii
5.4.2.12 tetramer 4 * 45300, deduced from nucleotide sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
5.4.2.12 iPGM i.e. 2,3-bisphosphoglycerate independent phosphoglycerate mutase Methanocaldococcus jannaschii
5.4.2.12 iPGM i.e. cofactor independent phosphoglycerate mutase Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.12 8
-
-
 Pyrococcus furiosus
5.4.2.12 8
-
-
 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.2.12 additional information 2,3-bisphosphoglycerate independent phosphoglycerate mutase Pyrococcus furiosus
5.4.2.12 additional information 2,3-bisphosphoglycerate independent phosphoglycerate mutase Methanocaldococcus jannaschii