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Literature summary extracted from

  • Moreno-Arribas, V.; Lonvaud-Funel, A.
    Purification and characterization of tyrosine decarboxylase of Lactobacillus brevis IOEB 9809 isolated from wine (2001), FEMS Microbiol. Lett., 195, 103-107.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.25 nutrition information about enzyme may contribute to the prevention of tyramine formation during wine production and storage Levilactobacillus brevis

General Stability

EC Number General Stability Organism
4.1.1.25 complete inactivation of purified TDC by freezing at -20°C, unstable during purification, washing of purified extract with sodium acetate buffer, pH 5, by ultracentrifugation results in complete loss of activity, partially restored by 0.2 mM pyridoxal 5’-phosphate Levilactobacillus brevis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.25 tyramine mixed non-competitive inhibitor Levilactobacillus brevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.25 0.63
-
 L-tyrosine pH 5, 25°C Levilactobacillus brevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.25 70000
-
 2 * 70000, SDS-PAGE Levilactobacillus brevis
4.1.1.25 140000
-
 gel filtration Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.25 L-Tyrosine Levilactobacillus brevis tyrosine decarboxylation in lactobacilli Tyramine + CO2
-
 ?
4.1.1.25 L-Tyrosine Levilactobacillus brevis IOEB 9809 tyrosine decarboxylation in lactobacilli Tyramine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.25 Levilactobacillus brevis
-
 isolated from wine
-
4.1.1.25 Levilactobacillus brevis IOEB 9809
-
 isolated from wine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.25 66fold Levilactobacillus brevis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.25 1058
-
 pH 5, 25°C Levilactobacillus brevis

Storage Stability

EC Number Storage Stability Organism
4.1.1.25 4°, 0.2 mM pyridoxal 5’-phosphate, 3 days, stable Levilactobacillus brevis
4.1.1.25 4°C, repeated storage, half-life: 1.5 days Levilactobacillus brevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.25 L-Tyrosine highly specific for L-tyrosine Levilactobacillus brevis Tyramine + CO2
-
 ?
4.1.1.25 L-Tyrosine tyrosine decarboxylation in lactobacilli Levilactobacillus brevis Tyramine + CO2
-
 ?
4.1.1.25 L-Tyrosine highly specific for L-tyrosine Levilactobacillus brevis IOEB 9809 Tyramine + CO2
-
 ?
4.1.1.25 L-Tyrosine tyrosine decarboxylation in lactobacilli Levilactobacillus brevis IOEB 9809 Tyramine + CO2
-
 ?
4.1.1.25 additional information not: histidine, lysine, phenylalanine, tryptophan, ornithine, L-DOPA Levilactobacillus brevis ?
-
 ?
4.1.1.25 additional information not: histidine, lysine, phenylalanine, tryptophan, ornithine, L-DOPA Levilactobacillus brevis IOEB 9809 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.25 homodimer 2 * 70000, SDS-PAGE Levilactobacillus brevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.25 25
-
 assay at Levilactobacillus brevis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.25 37
-
 stable up to for 10 min, after 20 min 75% loss of activity Levilactobacillus brevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.25 5
-
-
 Levilactobacillus brevis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.25 2 9 no activity below pH 2 or above pH 9 Levilactobacillus brevis
4.1.1.25 6
-
 more than 90% of maximum activity at pH 6 Levilactobacillus brevis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.25 pyridoxal 5'-phosphate coenzyme dependence Levilactobacillus brevis