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Literature summary extracted from

  • Bhatti, A.R.; Alvi, A.; Chaudhry, G.R.
    Evidence on the presence of two distinct alkaline phosphatases in Serratia marcescens (2000), FEMS Microbiol. Lett., 182, 131-135.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.1 NaCl 1 M, activation of enzyme form IAPase Serratia marcescens

General Stability

EC Number General Stability Organism
3.1.3.1 Mg2+ protects against thermal inactivation Serratia marcescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 NaCl 1 M, inhibition of enzyme form CAPase Serratia marcescens
3.1.3.1 phosphate competitive inhibition of both enzyme forms Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.0083
-
 4-nitrophenyl phosphate IAPase Serratia marcescens
3.1.3.1 0.057
-
 4-nitrophenyl phosphate CAPase Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Serratia marcescens
-
 2 distinct enzyme forms, an inducible enzyme form IAPase in 4 isozymes, and a constitutive enzyme form CAPase
-
3.1.3.1 Serratia marcescens 211
-
 2 distinct enzyme forms, an inducible enzyme form IAPase in 4 isozymes, and a constitutive enzyme form CAPase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1 2 distinct enzyme forms, CAPase 212fold and 4 isozymes of IAPase 5-87fold Serratia marcescens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.1 a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Serratia marcescens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 2.5
-
 purified isozyme IV of IAPase Serratia marcescens
3.1.3.1 22.2
-
 purified isozyme III of IAPase Serratia marcescens
3.1.3.1 30.8
-
 purified isozyme I of IAPase Serratia marcescens
3.1.3.1 43.5
-
 purified isozyme II of IAPase Serratia marcescens
3.1.3.1 106
-
 purified CAPase Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Serratia marcescens 4-nitrophenol + phosphate
-
 ?
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Serratia marcescens 211 4-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 alkaline phosphatase
-
 Serratia marcescens
3.1.3.1 APase
-
 Serratia marcescens
3.1.3.1 CAPase
-
 Serratia marcescens
3.1.3.1 IAPase
-
 Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 additional information
-
 Mg2+ protects against thermal inactivation up to 80°C Serratia marcescens
3.1.3.1 95
-
 5 min, in absence or presence of 10 mM Mg2+, inactivation of CAPase, while IApase is relatively stable showing loss of 30% activity after 5 min and loss of 80% activity after 30 min Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 8.5 10 IAPase Serratia marcescens
3.1.3.1 9.5
-
 CAPase Serratia marcescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.1 0.0053
-
 phosphate IAPase Serratia marcescens
3.1.3.1 0.76
-
 phosphate CAPase Serratia marcescens