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Literature summary extracted from

  • Miura, Y.; Gotoh, E.; Nara, F.; Nishijima, M.; Hanada, K.
    Hydrolysis of sphingosylphosphocholine by neutral sphingomyelinases (2004), FEBS Lett., 557, 288-292.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 phosphatidylserine 0.2 mM, approx. 108fold activation of sphingomyelin hydrolysis in the presence of 0.1% Triton X-100 Homo sapiens
3.1.4.12 Triton X-100 0.1%, approx. 108fold activation of sphingomyelin hydrolysis in the presence of 0.2 mM phosphatidylserine Homo sapiens
3.1.4.12 Triton X-100 0.1%, approx. 61fold activation of sphingomyelin hydrolysis in the presence of 0.2 mM phosphatidylserine Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12
-
 Bacillus cereus
3.1.4.12 expression of nSMase1 in HEK293 kidney cells Homo sapiens
3.1.4.12 expression of nSMase2 in HEK293 kidney cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 microsome
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Homo sapiens
-
 N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O Bacillus cereus
-
 N-acylsphingosine + choline phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Bacillus cereus
-
-
-
3.1.4.12 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 recombinant SMase Bacillus cereus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.12 0.46
-
 sphingosylphosphocholine hydrolysis Bacillus cereus
3.1.4.12 0.49
-
 sphingomyelin hydrolysis Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 lyso-platelet-activating factor + H2O 103fold higher activity than with sphingomyelin in the absence of detergent Homo sapiens 1-alkylglycerol + choline phosphate
-
 ?
3.1.4.12 lyso-platelet-activating factor + H2O 55% of activity with sphingomyelin in the absence of detergent Homo sapiens alkylglycerol + choline phosphate
-
 ?
3.1.4.12 lysophosphatidylcholine + H2O 198fold higher activity than with sphingomyelin in the absence of detergent Homo sapiens acylglycerol + choline phosphate
-
 ?
3.1.4.12 lysophosphatidylcholine + H2O 580% of activity with sphingomyelin in the absence of detergent Homo sapiens acylglycerol + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Homo sapiens N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Bacillus cereus N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O very low activity in the absence of detergent Homo sapiens N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingosylphosphocholine + H2O
-
 Bacillus cereus sphingosine + choline phosphate
-
 ?
3.1.4.12 sphingosylphosphocholine + H2O 127fold higher activity than with sphingomyelin in the absence of detergent Homo sapiens sphingosine + choline phosphate
-
 ?
3.1.4.12 sphingosylphosphocholine + H2O 980% of activity with sphingomyelin in the absence of detergent Homo sapiens sphingosine + choline phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 neutral sphingomyelinase
-
 Homo sapiens
3.1.4.12 neutral sphingomyelinase
-
 Bacillus cereus
3.1.4.12 nSMase
-
 Bacillus cereus
3.1.4.12 nSMase1
-
 Homo sapiens
3.1.4.12 nSMase2
-
 Homo sapiens