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Literature summary extracted from

  • Nunez, H.; Lefimil, C.; Min, B.; Soll, D.; Orellana, O.
    In vivo formation of glutamyl-tRNA(Gln) in Escherichia coli by heterologous glutamyl-tRNA synthetases (2004), FEBS Lett., 557, 133-135.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.17 expression in Escherichia coli Acidithiobacillus ferrooxidans
6.1.1.17 gene gltX mutant, mutant enzyme expression in Escherichia coli temperature-sensitive mutant strain Bacillus subtilis
6.1.1.17 gene gltX1 and gltX2, expression in Escherichia coli temperature-sensitive mutant strain Acidithiobacillus ferrooxidans
6.1.1.17 gene gltX2, expression in Escherichia coli temperature-sensitive mutant strain Helicobacter pylori
6.1.1.24 expression in Escherichia coli Acidithiobacillus ferrooxidans
6.1.1.24 expression in Escherichia coli Helicobacter pylori

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.17 Q373R substrate specificity restricted to tRNAGlu compared to the wild-type which also accepts tRNAGln Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGln Helicobacter pylori enzyme expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGln
-
?
6.1.1.17 ATP + L-glutamate + tRNAGln Acidithiobacillus ferrooxidans isozyme 2 expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGln
-
?
6.1.1.17 ATP + L-glutamate + tRNAGlu Helicobacter pylori
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
6.1.1.17 ATP + L-glutamate + tRNAGlu Acidithiobacillus ferrooxidans isozyme 1 expressed in an Escherichia coli mutant strain, tRNAGlu from Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
6.1.1.17 ATP + L-glutamate + tRNAGlu Bacillus subtilis recombinant mutant Q373R expressed in Escherichia coli mutant strain, tRNAGlu from Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
6.1.1.17 additional information Bacillus subtilis no charging of Escherichia coli tRNAGln by enzyme mutant Q373R ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.17 Acidithiobacillus ferrooxidans
-
-
-
6.1.1.17 Acidithiobacillus ferrooxidans
-
strain ATCC 23270, isoform 1 and 2, genes gltX1 and gltX2
-
6.1.1.17 Bacillus subtilis
-
-
-
6.1.1.17 Helicobacter pylori
-
isozyme 2
-
6.1.1.24 Acidithiobacillus ferrooxidans
-
-
-
6.1.1.24 Helicobacter pylori
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.17 ATP + Glu + tRNAGln
-
Acidithiobacillus ferrooxidans AMP + diphosphate + L-glutamyl-tRNAGln
-
?
6.1.1.17 ATP + L-glutamate + tRNAGln enzyme expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli Helicobacter pylori AMP + diphosphate + L-glutamyl-tRNAGln
-
?
6.1.1.17 ATP + L-glutamate + tRNAGln isozyme 2 expressed in an Escherichia coli mutant strain, tRNAGln UUG from Escherichia coli Acidithiobacillus ferrooxidans AMP + diphosphate + L-glutamyl-tRNAGln
-
?
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
Helicobacter pylori AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
6.1.1.17 ATP + L-glutamate + tRNAGlu isozyme 1 expressed in an Escherichia coli mutant strain, tRNAGlu from Escherichia coli Acidithiobacillus ferrooxidans AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
6.1.1.17 ATP + L-glutamate + tRNAGlu recombinant mutant Q373R expressed in Escherichia coli mutant strain, tRNAGlu from Escherichia coli Bacillus subtilis AMP + diphosphate + L-glutamyl-tRNAGlu
-
?
6.1.1.17 additional information no charging of Escherichia coli tRNAGln by enzyme mutant Q373R Bacillus subtilis ?
-
?
6.1.1.24 ATP + Glu + tRNAGln
-
Acidithiobacillus ferrooxidans AMP + diphosphate + L-glutamyl-tRNAGln
-
?
6.1.1.24 ATP + Glu + tRNAGln
-
Helicobacter pylori AMP + diphosphate + L-glutamyl-tRNAGln
-
?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.17 GluRS
-
Acidithiobacillus ferrooxidans
6.1.1.17 GluRS
-
Bacillus subtilis
6.1.1.17 GluRS
-
Helicobacter pylori
6.1.1.17 Glutamyl-tRNA synthetase
-
Acidithiobacillus ferrooxidans
6.1.1.17 Glutamyl-tRNA synthetase
-
Bacillus subtilis
6.1.1.17 Glutamyl-tRNA synthetase
-
Helicobacter pylori
6.1.1.24 GluRS2
-
Acidithiobacillus ferrooxidans
6.1.1.24 GluRS2
-
Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.17 ATP
-
Acidithiobacillus ferrooxidans
6.1.1.17 ATP
-
Bacillus subtilis
6.1.1.17 ATP
-
Helicobacter pylori