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Literature summary extracted from

  • Shenoy, A.R.; Srinivasan, N.; Subramaniam, M.; Visweswariah, S.S.
    Mutational analysis of the Mycobacterium tuberculosis Rv1625c adenylyl cyclase: residues that confer nucleotide specificity contribute to dimerization (2003), FEBS Lett., 545, 253-259.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.1 expression of cytosolic catalytic domain in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.1 D365C 0.06% of wild-type activity, mutant displays classical Michaelis-Menten kinetics, in contrast to the sigmoidal kinetics of the wild-type Mycobacterium tuberculosis
4.6.1.1 K296E 0.5% of wild-type activity, mutant displays classical Michaelis-Menten kinetics, in contrast to the sigmoidal kinetics of the wild-type Mycobacterium tuberculosis
4.6.1.1 K296E/D365C 0.025% of wild-type activity, mutant displays classical Michaelis-Menten kinetics, in contrast to the sigmoidal kinetics of the wild-type Mycobacterium tuberculosis
4.6.1.1 K296E/F363R/D365C 0.5% of wild-type activity, mutant displays classical Michaelis-Menten kinetics, in contrast to the sigmoidal kinetics of the wild-type Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.1 membrane 6 transmembrane helices and a single cytosolic catalytic domain, which dimerizes to form a 12-transmembrane homodimeric enzyme Mycobacterium tuberculosis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.1 Mn2+
-
 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.1 ATP Mycobacterium tuberculosis
-
 3',5'-cyclic AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.1 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.1 recombinant cytosolic catalytic domain Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.1 ATP
-
 Mycobacterium tuberculosis 3',5'-cyclic AMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.1 adenylyl cyclase
-
 Mycobacterium tuberculosis
4.6.1.1 Ry1625c
-
 Mycobacterium tuberculosis