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Literature summary extracted from

  • Fusi, P.; Villa, M.; Burlini, N.; Tortora, P.; Guerritore, A.
    Intracellular proteases from the extremely thermophilic archaebacterium Sulfolobus solfataricus (1991), Experientia, 47, 1057-1060.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.17.B1 leupeptin activates Saccharolobus solfataricus
3.4.17.B1 additional information not affected by EDTA, pepstatin, and bestatin, enzyme activity is increased 3 to 4fold when yeast extract instead of glucose is used in the cell culture medium Saccharolobus solfataricus
3.4.17.B1 phosphoramidon activates Saccharolobus solfataricus
3.4.17.B1 PMSF activates Saccharolobus solfataricus
3.4.17.B1 tosyl-lysine chloromethyl ketone activates Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.B1 additional information not affected by EDTA, pepstatin, and bestatin Saccharolobus solfataricus
3.4.17.B1 o-phenanthroline
-
 Saccharolobus solfataricus
3.4.17.B1 p-(chloromercuri)benzene sulfonate
-
 Saccharolobus solfataricus
3.4.21.B52 chymostatin 0.25 mg/ml, complete inhibition Saccharolobus solfataricus
3.4.21.B52 N-alpha-p-tosyl-L-phenylalanine chloromethyl ketone 0.5 mM, complete inhibition Saccharolobus solfataricus
3.4.21.B52 p-chloromercuribenzenesulfonate 10 mM, 13% inhibition Saccharolobus solfataricus
3.4.21.B52 phenylmethanesulfonyl fluoride 1 mM, 93% inhibition Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.B52 intracellular
-
 Saccharolobus solfataricus 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.B1 160000
-
 gel filtration Saccharolobus solfataricus
3.4.21.B52 115000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.B1 peptide + H2O Saccharolobus solfataricus
-
 ?
-
 ?
3.4.17.B1 peptide + H2O Saccharolobus solfataricus MT-4 / DSM 5833
-
 ?
-
 ?
3.4.17.B1 protein + H2O Saccharolobus solfataricus
-
 peptides
-
 ?
3.4.17.B1 protein + H2O Saccharolobus solfataricus MT-4 / DSM 5833
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.B1 Saccharolobus solfataricus
-
-
-
3.4.17.B1 Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-
3.4.21.B52 Saccharolobus solfataricus
-
-
-
3.4.21.B52 Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.B1 partial Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.B1 0.022
-
 crude cell extract, cells grown on glucose, exponential phase Saccharolobus solfataricus
3.4.17.B1 0.029
-
 crude cell extract, cells grown on glucose, stationary phase Saccharolobus solfataricus
3.4.17.B1 0.079
-
 crude cell extract, cells grown on yeast extract, stationary phase Saccharolobus solfataricus
3.4.17.B1 0.095
-
 crude cell extract, cells grown on yeast extract, exponential phase Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.B1 benzyloxycarbonyl-Asp + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 benzyloxycarbonyl-Asp + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
 ?
3.4.17.B1 peptide + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.17.B1 peptide + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
 ?
3.4.17.B1 protein + H2O
-
 Saccharolobus solfataricus peptides
-
 ?
3.4.17.B1 protein + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 peptides
-
 ?
3.4.21.B52 Suc-Phe-4-nitroanilide + H2O
-
 Saccharolobus solfataricus Suc-Phe + 4-nitroaniline
-
 ?
3.4.21.B52 Suc-Phe-4-nitroanilide + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 Suc-Phe + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.17.B1 M20.008 Merops-ID Saccharolobus solfataricus
3.4.21.B52 proteinase I
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B52 70
-
 assay at Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.17.B1 90
-
 stable at, 15 min, pH 7.0 Saccharolobus solfataricus
3.4.21.B52 90
-
 15 min, stable Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B52 7
-
 assay at Saccharolobus solfataricus