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Literature summary extracted from

  • Nanjundan, M.; Possmayer, F.
    Characterization of the pulmonary N-ethylmaleimide-insensitive phosphatidate phosphohydrolase (2000), Exp. Lung Res., 26, 361-381.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.4 Ca2+ 10 mM, 60% inhibition of PAP-2 Rattus norvegicus
3.1.3.4 ceramide 1-phosphate
-
 Rattus norvegicus
3.1.3.4 chlorpromazine 55% inhibition at equimolar concentration of substrate, PAP-2 Rattus norvegicus
3.1.3.4 diacylglycerol
-
 Rattus norvegicus
3.1.3.4 F- PAP-2 Rattus norvegicus
3.1.3.4 lysophosphatidic acid
-
 Rattus norvegicus
3.1.3.4 Mg2+ 15 mM MgCl2, 10-20% inhibition of PAP-2 Rattus norvegicus
3.1.3.4 monoacylglycerol
-
 Rattus norvegicus
3.1.3.4 NEM 2 mM, complete inhibition of PAP-1. NEM, up to 8 mM has little effect on PAP-2 Rattus norvegicus
3.1.3.4 spermidine trihydrochloride 7% inhibition at equimolar concentration of substrate, PAP-2 Rattus norvegicus
3.1.3.4 spermine 55% inhibition at equimolar concentration of substrate, PAP-2 Rattus norvegicus
3.1.3.4 sphingomyelin 72% inhibition at equimolar concentration with pure phosphatidic acid. No effect with equimolar concentrations of phosphatidic acid mixed with phosphatidylcholine Rattus norvegicus
3.1.3.4 sphingosine 93% inhibition at equimolar concentration with pure phosphatidic acid. 29% inhibition with equimolar concentrations of phosphatidic acid mixed with phosphatidylcholine Rattus norvegicus
3.1.3.4 sphingosine 1-phosphate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.4 plasma membrane
-
 Rattus norvegicus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.4 Mg2+ PAP-1 is dependent on Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.4 additional information Rattus norvegicus key enzyme involved in glycerolipid synthesis where it converts phosphatidic acid to diacylglycerol. PAP-1 is involved in phospholipid biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.4 Rattus norvegicus
-
 2 different phosphatidate phosphatidases: PAP-1 and PAP-2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.4 alveolar cell type II PAP-2 Rattus norvegicus
-
3.1.3.4 lung PAP-2 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.4 lysophosphatidic acid + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.3.4 additional information key enzyme involved in glycerolipid synthesis where it converts phosphatidic acid to diacylglycerol. PAP-1 is involved in phospholipid biosynthesis Rattus norvegicus ?
-
 ?
3.1.3.4 phosphatidic acid + H2O phosphatidic acid pure or in mixed micelles with phosphatidylcholine Rattus norvegicus 1,2-diacyl-sn-glycerol + phosphate
-
 ?
3.1.3.4 sphingosine 1-phosphate + H2O poor substrate Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.4 PAP-1
-
 Rattus norvegicus
3.1.3.4 PAP-2
-
 Rattus norvegicus
3.1.3.4 PAPase
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.4 55
-
 activity of PAP-2 is not seriously affected after 20 min Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.4 6
-
 phosphatidic acid mixed with phosphatidylcholine, PAP-2 Rattus norvegicus
3.1.3.4 6.5
-
 pure phosphatidic acid, PAP-2 Rattus norvegicus