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Literature summary extracted from

  • Hugler, M.; Krieger, R.S.; Jahn, M.; Fuchs, G.
    Characterization of acetyl-CoA/propionyl-CoA carboxylase in Metallosphaera sedula. Carboxylating enzyme in the 3-hydroxypropionate cycle for autotrophic carbon fixation (2003), Eur. J. Biochem., 270, 736-744.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.2 each subunit individually cloned, carboxyl transferase expressed in Escherichia coli Metallosphaera sedula
6.4.1.3 expressed in Escherichia coli Metallosphaera sedula

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 ADP
-
Metallosphaera sedula
6.4.1.2 Avidin reversible by addition of biotin Metallosphaera sedula
6.4.1.2 CoA
-
Metallosphaera sedula
6.4.1.2 malonyl-CoA
-
Metallosphaera sedula
6.4.1.2 succinyl-CoA
-
Metallosphaera sedula
6.4.1.3 ADP weak inhibition Metallosphaera sedula
6.4.1.3 CoA activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA Metallosphaera sedula
6.4.1.3 malonyl-CoA activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA Metallosphaera sedula
6.4.1.3 succinyl-CoA activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA Metallosphaera sedula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 0.04
-
ATP pH 7.5, 65°C Metallosphaera sedula
6.4.1.2 0.06
-
acetyl-CoA pH 7.5, 65°C Metallosphaera sedula
6.4.1.2 0.3
-
HCO3- pH 7.5, 65°C Metallosphaera sedula
6.4.1.3 0.04
-
ATP pH 7.5, 65°C Metallosphaera sedula
6.4.1.3 0.06
-
acetyl-CoA pH 7.5, 65°C Metallosphaera sedula
6.4.1.3 0.07
-
propanoyl-CoA pH 7.5, 65°C Metallosphaera sedula
6.4.1.3 0.3
-
HCO3- pH 7.5, 65°C Metallosphaera sedula

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.2 Ca2+ can partially replace for Mg2+ Metallosphaera sedula
6.4.1.2 Co2+ can partially replace for Mg2+ Metallosphaera sedula
6.4.1.2 Mg2+ required to form a complex with ATP Metallosphaera sedula
6.4.1.2 Mn2+ can partially replace for Mg2+ Metallosphaera sedula
6.4.1.3 Ca2+ 10% of the activity with Mg2+ Metallosphaera sedula
6.4.1.3 Co2+ 40% of the activity with Mg2+ Metallosphaera sedula
6.4.1.3 Mg2+ required for the formation of Mg-ATP complex, optimum concentration 5 mM at 5 mM ATP Metallosphaera sedula
6.4.1.3 Mn2+ 10% of the activity with Mg2+ Metallosphaera sedula

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 560000
-
gel filtration Metallosphaera sedula
6.4.1.3 18600
-
alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit) Metallosphaera sedula
6.4.1.3 57000
-
alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit) Metallosphaera sedula
6.4.1.3 560000
-
gel filtration Metallosphaera sedula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3- Chloroflexus aurantiacus no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3- Sulfuracidifex metallicus no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3- Metallosphaera sedula no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3- Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3- Chloroflexus aurantiacus OK-70-fl / DSM 636 no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation ADP + phosphate + malonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3- Chloroflexus aurantiacus CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3- Metallosphaera sedula CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3- Sulfuracidifex metallicus CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3- Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3- Chloroflexus aurantiacus OK-70-fl / DSM 636 CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle ADP + phosphate + (S)-methylmalonyl-CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Chloroflexus aurantiacus
-
-
-
6.4.1.2 Chloroflexus aurantiacus OK-70-fl / DSM 636
-
-
-
6.4.1.2 Metallosphaera sedula Q8J2Z4
-
-
6.4.1.2 Metallosphaera sedula Q8J2Z5
-
-
6.4.1.2 Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 Q8J2Z4
-
-
6.4.1.2 Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 Q8J2Z5
-
-
6.4.1.2 Sulfuracidifex metallicus
-
DSM 6482
-
6.4.1.3 Chloroflexus aurantiacus
-
-
-
6.4.1.3 Chloroflexus aurantiacus OK-70-fl / DSM 636
-
-
-
6.4.1.3 Metallosphaera sedula
-
-
-
6.4.1.3 Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348
-
-
-
6.4.1.3 Sulfuracidifex metallicus
-
DSM 6482
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2
-
Metallosphaera sedula
6.4.1.2 partial Chloroflexus aurantiacus
6.4.1.3
-
Metallosphaera sedula

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 3.2
-
purified enzyme, pH 7.5, 65°C Metallosphaera sedula
6.4.1.3 3.3
-
purified enzyme, pH 7.5, 65°C Metallosphaera sedula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
Chloroflexus aurantiacus ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
Metallosphaera sedula ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
Sulfuracidifex metallicus ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3- no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation Chloroflexus aurantiacus ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3- no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation Sulfuracidifex metallicus ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3- no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation Metallosphaera sedula ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3- no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
Chloroflexus aurantiacus OK-70-fl / DSM 636 ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + acetyl-CoA + HCO3- no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation Chloroflexus aurantiacus OK-70-fl / DSM 636 ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + propionyl-CoA + HCO3- second activity of the protein representing EC 6.4.1.3 Metallosphaera sedula ADP + phosphate + methylmalonyl-CoA
-
?
6.4.1.2 ATP + propionyl-CoA + HCO3- second activity of the protein representing EC 6.4.1.3 Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 ADP + phosphate + methylmalonyl-CoA
-
?
6.4.1.2 GTP + acetyl-CoA + HCO3- 10% of the activity obtained with ATP Metallosphaera sedula GDP + phosphate + malonyl-CoA
-
?
6.4.1.2 GTP + acetyl-CoA + HCO3- 10% of the activity obtained with ATP Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 GDP + phosphate + malonyl-CoA
-
?
6.4.1.2 UTP + acetyl-CoA + HCO3- 40% of the activity obtained with ATP Metallosphaera sedula UDP + phosphate + malonyl-CoA
-
?
6.4.1.2 UTP + acetyl-CoA + HCO3- 40% of the activity obtained with ATP Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 UDP + phosphate + malonyl-CoA
-
?
6.4.1.3 ATP + acetyl-CoA + HCO3- EC 6.4.1.2 activity Chloroflexus aurantiacus ADP + phosphate + malonyl-CoA
-
?
6.4.1.3 ATP + acetyl-CoA + HCO3- EC 6.4.1.2 activity Metallosphaera sedula ADP + phosphate + malonyl-CoA
-
?
6.4.1.3 ATP + acetyl-CoA + HCO3- EC 6.4.1.2 activity Sulfuracidifex metallicus ADP + phosphate + malonyl-CoA
-
?
6.4.1.3 ATP + acetyl-CoA + HCO3- EC 6.4.1.2 activity Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 ADP + phosphate + malonyl-CoA
-
?
6.4.1.3 ATP + acetyl-CoA + HCO3- EC 6.4.1.2 activity Chloroflexus aurantiacus OK-70-fl / DSM 636 ADP + phosphate + malonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
Chloroflexus aurantiacus ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
Metallosphaera sedula ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
Sulfuracidifex metallicus ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3- CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle Chloroflexus aurantiacus ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3- CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle Metallosphaera sedula ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3- CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle Sulfuracidifex metallicus ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3- CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3-
-
Chloroflexus aurantiacus OK-70-fl / DSM 636 ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 ATP + propanoyl-CoA + HCO3- CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle Chloroflexus aurantiacus OK-70-fl / DSM 636 ADP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 GTP + propanoyl-CoA + HCO3- 10% of the activity with ATP Metallosphaera sedula GDP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 GTP + propanoyl-CoA + HCO3- 10% of the activity with ATP Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 GDP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 UTP + propanoyl-CoA + HCO3- 40% of the activity with ATP Metallosphaera sedula UDP + phosphate + (S)-methylmalonyl-CoA
-
?
6.4.1.3 UTP + propanoyl-CoA + HCO3- 40% of the activity with ATP Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 UDP + phosphate + (S)-methylmalonyl-CoA
-
?

Subunits

EC Number Subunits Comment Organism
6.4.1.2 multimer most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit) Chloroflexus aurantiacus
6.4.1.2 multimer most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit) Sulfuracidifex metallicus
6.4.1.2 multimer most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit) Metallosphaera sedula
6.4.1.3 dodecamer alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit) Metallosphaera sedula

Synonyms

EC Number Synonyms Comment Organism
6.4.1.2 acetyl-CoA/propionyl-CoA carboxylase enzyme acts on both substrates Chloroflexus aurantiacus
6.4.1.2 acetyl-CoA/propionyl-CoA carboxylase enzyme acts on both substrates Sulfuracidifex metallicus
6.4.1.2 acetyl-CoA/propionyl-CoA carboxylase enzyme acts on both substrates Metallosphaera sedula
6.4.1.3 acetyl-CoA/propionyl-CoA carboxylase enzyme acts equally well on both substrates Chloroflexus aurantiacus
6.4.1.3 acetyl-CoA/propionyl-CoA carboxylase enzyme acts equally well on both substrates Metallosphaera sedula
6.4.1.3 acetyl-CoA/propionyl-CoA carboxylase enzyme acts equally well on both substrates Sulfuracidifex metallicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.4.1.2 75
-
-
Metallosphaera sedula
6.4.1.3 75
-
-
Metallosphaera sedula

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.4.1.2 40 85
-
Metallosphaera sedula
6.4.1.3 40 85
-
Metallosphaera sedula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.4.1.2 2 8 acetyl-CoA pH 7.5, 65°C Metallosphaera sedula
6.4.1.3 2 8 propanoyl-CoA pH 7.5, 65°C Metallosphaera sedula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 7.5
-
-
Metallosphaera sedula
6.4.1.3 7.5
-
-
Metallosphaera sedula

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.4.1.2 6 8
-
Metallosphaera sedula
6.4.1.3 6 8.5
-
Metallosphaera sedula

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.2 biotin enzyme-bound Chloroflexus aurantiacus
6.4.1.2 biotin enzyme-bound Sulfuracidifex metallicus
6.4.1.2 biotin enzyme-bound Metallosphaera sedula
6.4.1.3 biotin bound to enzyme Chloroflexus aurantiacus
6.4.1.3 biotin bound to enzyme Metallosphaera sedula
6.4.1.3 biotin bound to enzyme Sulfuracidifex metallicus