EC Number | Cloned (Comment) | Organism |
---|---|---|
6.4.1.2 | each subunit individually cloned, carboxyl transferase expressed in Escherichia coli | Metallosphaera sedula |
6.4.1.3 | expressed in Escherichia coli | Metallosphaera sedula |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.2 | ADP | - |
Metallosphaera sedula | |
6.4.1.2 | Avidin | reversible by addition of biotin | Metallosphaera sedula | |
6.4.1.2 | CoA | - |
Metallosphaera sedula | |
6.4.1.2 | malonyl-CoA | - |
Metallosphaera sedula | |
6.4.1.2 | succinyl-CoA | - |
Metallosphaera sedula | |
6.4.1.3 | ADP | weak inhibition | Metallosphaera sedula | |
6.4.1.3 | CoA | activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA | Metallosphaera sedula | |
6.4.1.3 | malonyl-CoA | activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA | Metallosphaera sedula | |
6.4.1.3 | succinyl-CoA | activity on propanoyl-CoA is more strongly inhibited than activity of acetyl-CoA | Metallosphaera sedula |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.2 | 0.04 | - |
ATP | pH 7.5, 65°C | Metallosphaera sedula | |
6.4.1.2 | 0.06 | - |
acetyl-CoA | pH 7.5, 65°C | Metallosphaera sedula | |
6.4.1.2 | 0.3 | - |
HCO3- | pH 7.5, 65°C | Metallosphaera sedula | |
6.4.1.3 | 0.04 | - |
ATP | pH 7.5, 65°C | Metallosphaera sedula | |
6.4.1.3 | 0.06 | - |
acetyl-CoA | pH 7.5, 65°C | Metallosphaera sedula | |
6.4.1.3 | 0.07 | - |
propanoyl-CoA | pH 7.5, 65°C | Metallosphaera sedula | |
6.4.1.3 | 0.3 | - |
HCO3- | pH 7.5, 65°C | Metallosphaera sedula |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.2 | Ca2+ | can partially replace for Mg2+ | Metallosphaera sedula | |
6.4.1.2 | Co2+ | can partially replace for Mg2+ | Metallosphaera sedula | |
6.4.1.2 | Mg2+ | required to form a complex with ATP | Metallosphaera sedula | |
6.4.1.2 | Mn2+ | can partially replace for Mg2+ | Metallosphaera sedula | |
6.4.1.3 | Ca2+ | 10% of the activity with Mg2+ | Metallosphaera sedula | |
6.4.1.3 | Co2+ | 40% of the activity with Mg2+ | Metallosphaera sedula | |
6.4.1.3 | Mg2+ | required for the formation of Mg-ATP complex, optimum concentration 5 mM at 5 mM ATP | Metallosphaera sedula | |
6.4.1.3 | Mn2+ | 10% of the activity with Mg2+ | Metallosphaera sedula |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.4.1.2 | 560000 | - |
gel filtration | Metallosphaera sedula |
6.4.1.3 | 18600 | - |
alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit) | Metallosphaera sedula |
6.4.1.3 | 57000 | - |
alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit) | Metallosphaera sedula |
6.4.1.3 | 560000 | - |
gel filtration | Metallosphaera sedula |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.2 | ATP + acetyl-CoA + HCO3- | Chloroflexus aurantiacus | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | Sulfuracidifex metallicus | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | Metallosphaera sedula | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | Chloroflexus aurantiacus OK-70-fl / DSM 636 | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | Chloroflexus aurantiacus | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | Metallosphaera sedula | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | Sulfuracidifex metallicus | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | Chloroflexus aurantiacus OK-70-fl / DSM 636 | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.4.1.2 | Chloroflexus aurantiacus | - |
- |
- |
6.4.1.2 | Chloroflexus aurantiacus OK-70-fl / DSM 636 | - |
- |
- |
6.4.1.2 | Metallosphaera sedula | Q8J2Z4 | - |
- |
6.4.1.2 | Metallosphaera sedula | Q8J2Z5 | - |
- |
6.4.1.2 | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | Q8J2Z4 | - |
- |
6.4.1.2 | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | Q8J2Z5 | - |
- |
6.4.1.2 | Sulfuracidifex metallicus | - |
DSM 6482 | - |
6.4.1.3 | Chloroflexus aurantiacus | - |
- |
- |
6.4.1.3 | Chloroflexus aurantiacus OK-70-fl / DSM 636 | - |
- |
- |
6.4.1.3 | Metallosphaera sedula | - |
- |
- |
6.4.1.3 | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | - |
- |
- |
6.4.1.3 | Sulfuracidifex metallicus | - |
DSM 6482 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.4.1.2 | - |
Metallosphaera sedula |
6.4.1.2 | partial | Chloroflexus aurantiacus |
6.4.1.3 | - |
Metallosphaera sedula |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.4.1.2 | 3.2 | - |
purified enzyme, pH 7.5, 65°C | Metallosphaera sedula |
6.4.1.3 | 3.3 | - |
purified enzyme, pH 7.5, 65°C | Metallosphaera sedula |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.2 | ATP + acetyl-CoA + HCO3- | - |
Chloroflexus aurantiacus | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | - |
Metallosphaera sedula | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | - |
Sulfuracidifex metallicus | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Chloroflexus aurantiacus | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Sulfuracidifex metallicus | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Metallosphaera sedula | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | - |
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | - |
Chloroflexus aurantiacus OK-70-fl / DSM 636 | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + acetyl-CoA + HCO3- | no fatty acid synthesis, involved in 3-hydroxypropionate cycle for autotrophic CO2 fixation | Chloroflexus aurantiacus OK-70-fl / DSM 636 | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | ATP + propionyl-CoA + HCO3- | second activity of the protein representing EC 6.4.1.3 | Metallosphaera sedula | ADP + phosphate + methylmalonyl-CoA | - |
? | |
6.4.1.2 | ATP + propionyl-CoA + HCO3- | second activity of the protein representing EC 6.4.1.3 | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + methylmalonyl-CoA | - |
? | |
6.4.1.2 | GTP + acetyl-CoA + HCO3- | 10% of the activity obtained with ATP | Metallosphaera sedula | GDP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | GTP + acetyl-CoA + HCO3- | 10% of the activity obtained with ATP | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | GDP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | UTP + acetyl-CoA + HCO3- | 40% of the activity obtained with ATP | Metallosphaera sedula | UDP + phosphate + malonyl-CoA | - |
? | |
6.4.1.2 | UTP + acetyl-CoA + HCO3- | 40% of the activity obtained with ATP | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | UDP + phosphate + malonyl-CoA | - |
? | |
6.4.1.3 | ATP + acetyl-CoA + HCO3- | EC 6.4.1.2 activity | Chloroflexus aurantiacus | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.3 | ATP + acetyl-CoA + HCO3- | EC 6.4.1.2 activity | Metallosphaera sedula | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.3 | ATP + acetyl-CoA + HCO3- | EC 6.4.1.2 activity | Sulfuracidifex metallicus | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.3 | ATP + acetyl-CoA + HCO3- | EC 6.4.1.2 activity | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.3 | ATP + acetyl-CoA + HCO3- | EC 6.4.1.2 activity | Chloroflexus aurantiacus OK-70-fl / DSM 636 | ADP + phosphate + malonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | - |
Chloroflexus aurantiacus | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | - |
Metallosphaera sedula | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | - |
Sulfuracidifex metallicus | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | Chloroflexus aurantiacus | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | Metallosphaera sedula | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | Sulfuracidifex metallicus | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | - |
Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | - |
Chloroflexus aurantiacus OK-70-fl / DSM 636 | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | ATP + propanoyl-CoA + HCO3- | CO2 fixation during autotrophic growth, 3 -hydroxypropionate cycle | Chloroflexus aurantiacus OK-70-fl / DSM 636 | ADP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | GTP + propanoyl-CoA + HCO3- | 10% of the activity with ATP | Metallosphaera sedula | GDP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | GTP + propanoyl-CoA + HCO3- | 10% of the activity with ATP | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | GDP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | UTP + propanoyl-CoA + HCO3- | 40% of the activity with ATP | Metallosphaera sedula | UDP + phosphate + (S)-methylmalonyl-CoA | - |
? | |
6.4.1.3 | UTP + propanoyl-CoA + HCO3- | 40% of the activity with ATP | Metallosphaera sedula TH2 / ATCC 51363 / DSM 5348 | UDP + phosphate + (S)-methylmalonyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.4.1.2 | multimer | most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit) | Chloroflexus aurantiacus |
6.4.1.2 | multimer | most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit) | Sulfuracidifex metallicus |
6.4.1.2 | multimer | most likely (alpha,beta,gamma)4 composition, two large subunits of 57000 Da: biotin carboxylase (alpha subunit) and carboxyl transferase (gamma subunit), 18600 Da biotin carrier protein (beta subunit) | Metallosphaera sedula |
6.4.1.3 | dodecamer | alpha, beta, gamma, 4 * 57000 + 4 * 57000 + 4 * 18600, SDS-PAGE, biotin carboxylase (alpha subunit), carboxyl transferase (beta subunit) and biotin carrier protein (gamma subunit) | Metallosphaera sedula |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.4.1.2 | acetyl-CoA/propionyl-CoA carboxylase | enzyme acts on both substrates | Chloroflexus aurantiacus |
6.4.1.2 | acetyl-CoA/propionyl-CoA carboxylase | enzyme acts on both substrates | Sulfuracidifex metallicus |
6.4.1.2 | acetyl-CoA/propionyl-CoA carboxylase | enzyme acts on both substrates | Metallosphaera sedula |
6.4.1.3 | acetyl-CoA/propionyl-CoA carboxylase | enzyme acts equally well on both substrates | Chloroflexus aurantiacus |
6.4.1.3 | acetyl-CoA/propionyl-CoA carboxylase | enzyme acts equally well on both substrates | Metallosphaera sedula |
6.4.1.3 | acetyl-CoA/propionyl-CoA carboxylase | enzyme acts equally well on both substrates | Sulfuracidifex metallicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.2 | 75 | - |
- |
Metallosphaera sedula |
6.4.1.3 | 75 | - |
- |
Metallosphaera sedula |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.4.1.2 | 40 | 85 | - |
Metallosphaera sedula |
6.4.1.3 | 40 | 85 | - |
Metallosphaera sedula |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.2 | 2 | 8 | acetyl-CoA | pH 7.5, 65°C | Metallosphaera sedula | |
6.4.1.3 | 2 | 8 | propanoyl-CoA | pH 7.5, 65°C | Metallosphaera sedula |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.4.1.2 | 7.5 | - |
- |
Metallosphaera sedula |
6.4.1.3 | 7.5 | - |
- |
Metallosphaera sedula |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.4.1.2 | 6 | 8 | - |
Metallosphaera sedula |
6.4.1.3 | 6 | 8.5 | - |
Metallosphaera sedula |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.2 | biotin | enzyme-bound | Chloroflexus aurantiacus | |
6.4.1.2 | biotin | enzyme-bound | Sulfuracidifex metallicus | |
6.4.1.2 | biotin | enzyme-bound | Metallosphaera sedula | |
6.4.1.3 | biotin | bound to enzyme | Chloroflexus aurantiacus | |
6.4.1.3 | biotin | bound to enzyme | Metallosphaera sedula | |
6.4.1.3 | biotin | bound to enzyme | Sulfuracidifex metallicus |