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Literature summary extracted from

  • Hong, S.W.; Kim, B.T.; Shin, H.Y.; Kim, W.S.; Lee, K.S.; Kim, Y.S.; Kim, D.H.
    Purification and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal anaerobic bacterium (2002), Eur. J. Biochem., 269, 2934-2940.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.5 additional information not affected by Mg2+, Mn2+, Ca2+, Co2+, and EDTA Bacteroides stercoris

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.5 Cu2+ strong inhibition Bacteroides stercoris
4.2.2.5 additional information not affected by Mg2+, Mn2+, Ca2+, Co2+, and EDTA Bacteroides stercoris
4.2.2.5 Ni2+
-
 Bacteroides stercoris
4.2.2.5 p-chloromercuriphenyl sulfonic acid strong inhibition Bacteroides stercoris
4.2.2.5 Zn2+ strong inhibition Bacteroides stercoris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.5 additional information
-
 additional information
-
 Bacteroides stercoris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.5 84000
-
 2 * 84000, SDS-PAGE Bacteroides stercoris
4.2.2.5 170000
-
 gel filtration Bacteroides stercoris

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.5 Bacteroides stercoris
-
 human intestinal anaerobic bacterium
-
4.2.2.5 Bacteroides stercoris HJ-15
-
 human intestinal anaerobic bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.5 to homogeneity Bacteroides stercoris

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.5 [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine]n = n-1 4-deoxy-beta-D-gluc-4-enuronosyl-1,4-beta-D-hexosylamine + [1,3-beta-D-glucuronic acid-1,4-beta-D-hexosylamine] acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate Bacteroides stercoris
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.5 57.03
-
 purified enzyme Bacteroides stercoris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.5 chondroitin sulfate A i.e. chondroitin 4-sulfate, best substrate Bacteroides stercoris ?
-
 ?
4.2.2.5 chondroitin sulfate A i.e. chondroitin 4-sulfate, best substrate Bacteroides stercoris HJ-15 ?
-
 ?
4.2.2.5 chondroitin sulfate C i.e. chondroitin 6-sulfate, low activity Bacteroides stercoris ?
-
 ?
4.2.2.5 chondroitin sulfate C i.e. chondroitin 6-sulfate, low activity Bacteroides stercoris HJ-15 ?
-
 ?
4.2.2.5 hyaluronic acid low activity Bacteroides stercoris ?
-
 ?
4.2.2.5 hyaluronic acid low activity Bacteroides stercoris HJ-15 ?
-
 ?
4.2.2.5 additional information no activity with dermatan sufate, heparin and heparan sulfate, the latter of porcine or bovine source Bacteroides stercoris ?
-
 ?
4.2.2.5 additional information no activity with dermatan sufate, heparin and heparan sulfate, the latter of porcine or bovine source Bacteroides stercoris HJ-15 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.5 dimer 2 * 84000, SDS-PAGE Bacteroides stercoris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.5 45 50
-
 Bacteroides stercoris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.5 5.7 6
-
 Bacteroides stercoris