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Literature summary extracted from

  • Suematsu, N.; Okamoto, K.; Shibata, K.; Nakanishi, Y.; Isohashi, F.
    Molecular cloning and functional expression of rat liver cytosolic acetyl-CoA hydrolase (2001), Eur. J. Biochem., 268, 2700-2709.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.1 expression in Sf9 cells Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.1 0.153
-
 acetyl-CoA 25°C, recombinant enzyme Rattus norvegicus
3.1.2.1 0.22
-
 acetyl-CoA 25°C, native enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.1 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.1 62000
-
 2 * 62000, SDS-PAGE Rattus norvegicus
3.1.2.1 62017
-
 2 * 62017, calculation from nucleotide sequence Rattus norvegicus
3.1.2.1 124000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.1 Rattus norvegicus Q99NB7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.1 329
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.1 acetyl-CoA + H2O
-
 Rattus norvegicus CoA + acetate
-
 ?
3.1.2.1 butanoyl-CoA + H2O about 20% of the activity with acetyl-CoA Rattus norvegicus CoA + butanoate
-
 ?
3.1.2.1 hexanoyl-CoA + H2O about 10% of the activity with acetyl-CoA Rattus norvegicus CoA + hexanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.1 dimer 2 * 62000, SDS-PAGE Rattus norvegicus
3.1.2.1 dimer 2 * 62017, calculation from nucleotide sequence Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.1.2.1 CACH
-
 Rattus norvegicus