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Literature summary extracted from

  • Lee, D.W.; Kim, H.W.; Lee, K.W.; Kim, B.C.; Choe, E.A.; Lee, H.S.; Kim, D.S.; Pyun, Y.R.
    Purification and characterization of two distinct thermostable lipases from the gram-positive thermophilic bacterium Bacillus thermoleovorans ID-1 (2001), Enzyme Microb. Technol., 29, 363-371.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 Ca2+ BTID-B, slight activation Geobacillus thermoleovorans
3.1.1.3 Co2+ BTID-B, slight activation Geobacillus thermoleovorans
3.1.1.3 K+ BTID-B, slight activation Geobacillus thermoleovorans
3.1.1.3 Mn2+ BTID-B, slight activation Geobacillus thermoleovorans
3.1.1.3 Na+ BTID-B, slight activation Geobacillus thermoleovorans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 BTID-B, DNA sequence determination and analysis, expression in Escherichia coli Geobacillus thermoleovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 2-mercaptoethanol BTID-A, 26% inhibition at 1% w/v Geobacillus thermoleovorans
3.1.1.3 Ag+ BTID-A Geobacillus thermoleovorans
3.1.1.3 Ca2+ BTID-A, slight inhibition Geobacillus thermoleovorans
3.1.1.3 Co2+ BTID-A Geobacillus thermoleovorans
3.1.1.3 Cu2+ BTID-A, BTID-B Geobacillus thermoleovorans
3.1.1.3 dithiothreitol BTID-A, 48% inhibition at 1% w/v Geobacillus thermoleovorans
3.1.1.3 DMSO BTID-A, 54% inhibition at 1% w/v Geobacillus thermoleovorans
3.1.1.3 EDTA BTID-B, 45% inhibition at 1% w/v, BTID-A, 68% inhibition at 1% w/v Geobacillus thermoleovorans
3.1.1.3 ethanol BTID-A, 42% inhibition at 1% w/v Geobacillus thermoleovorans
3.1.1.3 Fe2+ BTID-A, BTID-B Geobacillus thermoleovorans
3.1.1.3 Hg2+ BTID-A, BTID-B Geobacillus thermoleovorans
3.1.1.3 K+ BTID-A Geobacillus thermoleovorans
3.1.1.3 Mg2+ BTID-A Geobacillus thermoleovorans
3.1.1.3 Mn2+ BTID-A Geobacillus thermoleovorans
3.1.1.3 Na+ BTID-A Geobacillus thermoleovorans
3.1.1.3 PMSF BTID-A, 92% inhibition at 1% w/v Geobacillus thermoleovorans
3.1.1.3 SDS BTID-B, complete inhibition at 1% w/v, no inhibition of BTID-A Geobacillus thermoleovorans
3.1.1.3 Zn2+ BTID-A, BTID-B Geobacillus thermoleovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 1.82
-
 tricaprylin BTID-A, pH 7.5, 60°C Geobacillus thermoleovorans
3.1.1.3 6.24
-
 tricaprylin BTID-B, pH 7.5, 60°C Geobacillus thermoleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 18000
-
 x * 18000, BTID-A, SDS-PAGE, 1* 43000, BTID-B, SDS-PAGE Geobacillus thermoleovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 additional information Geobacillus thermoleovorans enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources ?
-
 ?
3.1.1.3 additional information Geobacillus thermoleovorans ID-1 enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 additional information BTID-B is strongly resistant to organic solvents Geobacillus thermoleovorans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Geobacillus thermoleovorans
-
 2 thermostable lipases A and B, BTID-A and BTID-B
-
3.1.1.3 Geobacillus thermoleovorans ID-1
-
 2 thermostable lipases A and B, BTID-A and BTID-B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 to homogeneity, BTID-A 300fold, recombinant BTID-B 107.5fold Geobacillus thermoleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 12
-
 purified BTID-A Geobacillus thermoleovorans
3.1.1.3 292
-
 purified recombinant BTID-B Geobacillus thermoleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 1,2,3-trioleoyl-glycerol + H2O
-
 Geobacillus thermoleovorans ?
-
 ?
3.1.1.3 1,2-dilinoleoyl-3-oleoyl-rac-glycerol + H2O
-
 Geobacillus thermoleovorans ?
-
 ?
3.1.1.3 1,3-dioleoyl-2-palmitoyl-glycerol + H2O not BTID-A Geobacillus thermoleovorans ?
-
 ?
3.1.1.3 1,3-dipalmitoyl-2-oleoyl-glycerol + H2O
-
 Geobacillus thermoleovorans ?
-
 ?
3.1.1.3 additional information enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources Geobacillus thermoleovorans ?
-
 ?
3.1.1.3 additional information enzyme hydrolyzes triacylglycerides of plant oils such as olive oil, soybean oil, palm oil, and lard as carbon sources Geobacillus thermoleovorans ID-1 ?
-
 ?
3.1.1.3 tributyrin + H2O not BTID-A Geobacillus thermoleovorans dibutyrin + butyrate
-
 ?
3.1.1.3 tributyrin + H2O not BTID-A Geobacillus thermoleovorans ID-1 dibutyrin + butyrate
-
 ?
3.1.1.3 tricaprin + H2O
-
 Geobacillus thermoleovorans dicaprin + decaprate
-
 ?
3.1.1.3 tricaproin + H2O not BTID-A Geobacillus thermoleovorans dicaproin + caproate
-
 ?
3.1.1.3 tricaprylin + H2O best substrate, BTID-A and BTID-B Geobacillus thermoleovorans dicaprylin + caprylate
-
 ?
3.1.1.3 tricaprylin + H2O best substrate, BTID-A and BTID-B Geobacillus thermoleovorans ID-1 dicaprylin + caprylate
-
 ?
3.1.1.3 trilaurin + H2O
-
 Geobacillus thermoleovorans dilaurin + laurate
-
 ?
3.1.1.3 trilaurin + H2O
-
 Geobacillus thermoleovorans ID-1 dilaurin + laurate
-
 ?
3.1.1.3 trimyristin + H2O
-
 Geobacillus thermoleovorans dimyristin + myristate
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Geobacillus thermoleovorans dipalmitin + palmitate
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Geobacillus thermoleovorans ID-1 dipalmitin + palmitate
-
 ?
3.1.1.3 tristearin + H2O
-
 Geobacillus thermoleovorans distearin + stearate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 18000, BTID-A, SDS-PAGE, 1* 43000, BTID-B, SDS-PAGE Geobacillus thermoleovorans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 BTID
-
 Geobacillus thermoleovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 60
-
 BTID-B Geobacillus thermoleovorans
3.1.1.3 60 65 BTID-A Geobacillus thermoleovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 60
-
 BTID-B is stable for 2 h, BTID-A shows over 75% remaining activity after 30 min Geobacillus thermoleovorans
3.1.1.3 70
-
 BTID-B, 30 min, 50% remaining activity Geobacillus thermoleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8 9 BTID-B Geobacillus thermoleovorans
3.1.1.3 9
-
 BTID-A Geobacillus thermoleovorans