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Literature summary extracted from
- Hydroxynitrile lyases in stereoselective catalysis (2000), Curr. Opin. Biotechnol., 11, 532-539.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.1.2.10 | synthesis | used as a catalyst in the preparation of optically active cyanohydrins | Prunus sp. |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.2.47 | succesfully overexpressed in Escherichia coli, Saccharomyces cerevisiae and Pichia pastoris | Manihot esculenta |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.10 | 55000 | 80000 | - |
Prunus sp. |
| 4.1.2.11 | 22000 | - |
x * 33000 + x * 22000 | Sorghum bicolor |
| 4.1.2.11 | 33000 | - |
x * 33000 + x * 22000 | Sorghum bicolor |
| 4.1.2.11 | 105000 | - |
- |
Sorghum bicolor |
| 4.1.2.47 | 42000 | - |
2 * 42000 | Linum usitatissimum |
| 4.1.2.47 | 82000 | - |
- |
Linum usitatissimum |
| 4.1.2.47 | 92000 | 124000 | - |
Manihot esculenta |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.10 | cyanide + benzaldehyde | Prunus sp. | - |
(R)-mandelonitrile | - |
r |  |
| 4.1.2.11 | HCN + 4-hydroxybenzaldehyde | Sorghum bicolor | - |
(S)-p-hydroxymandelonitrile | - |
? | |
| 4.1.2.47 | Acetone cyanhydrin | Manihot esculenta | - |
Cyanide + acetone | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.10 | Prunus sp. | - |
- |
- |
| 4.1.2.11 | Sorghum bicolor | - |
- |
- |
| 4.1.2.47 | Linum usitatissimum | - |
flax | - |
| 4.1.2.47 | Manihot esculenta | - |
maniot | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.10 | cyanide + benzaldehyde | - |
Prunus sp. | (R)-mandelonitrile | - |
r | |
| 4.1.2.10 | cyanide + benzaldehyde | (R)-specific | Prunus sp. | (R)-mandelonitrile | - |
? | |
| 4.1.2.11 | HCN + 4-hydroxybenzaldehyde | (S)-specific | Sorghum bicolor | (S)-4-hydroxymandelonitrile | - |
? | |
| 4.1.2.11 | HCN + 4-hydroxybenzaldehyde | - |
Sorghum bicolor | (S)-p-hydroxymandelonitrile | - |
? | |
| 4.1.2.11 | additional information | substrate range of SbHNL is limited to aromatic and heteroaromatic aldehydes, does not accept aliphatic aldehydes as substrates | Sorghum bicolor | ? | - |
? | |
| 4.1.2.47 | Acetone cyanhydrin | - |
Manihot esculenta | Cyanide + acetone | - |
? | |
| 4.1.2.47 | HCN + (E)-2-butenal | - |
Manihot esculenta | (3E)-2-hydroxypent-3-enenitrile | - |
? | |
| 4.1.2.47 | HCN + 2,2-dimethylpropanal | - |
Manihot esculenta | 2-hydroxy-3,3-dimethylbutyronitrile | - |
? | |
| 4.1.2.47 | HCN + 2-chlorobenzaldehyde | - |
Manihot esculenta | (2-chlorophenyl)(hydroxy)acetonitrile | - |
? | |
| 4.1.2.47 | HCN + 2-hexenal | - |
Manihot esculenta | 2-hydroxyhept-3-enenitrile | - |
? | |
| 4.1.2.47 | HCN + 2-propenal | - |
Manihot esculenta | 2-hydroxybut-3-enenitrile | - |
? | |
| 4.1.2.47 | HCN + 3-furylcarbaldehyde | - |
Manihot esculenta | hydroxy(fur-3yl)acetonitrile | - |
? | |
| 4.1.2.47 | HCN + 4-methoxybenzaldehyde | - |
Manihot esculenta | (4-methoxyphenyl) (hydroxy)acetonitrile | - |
? | |
| 4.1.2.47 | HCN + benzaldehyde | - |
Linum usitatissimum | (R)-mandelonitrile | - |
? | |
| 4.1.2.47 | HCN + benzaldehyde | - |
Manihot esculenta | (R)-mandelonitrile | - |
? | |
| 4.1.2.47 | HCN + benzene-1,2,4-tricarbaldehyde | - |
Manihot esculenta | ? | - |
? | |
| 4.1.2.47 | HCN + isobutyraldehyde | - |
Manihot esculenta | 2-hydroxy-3-methylbutyronitrile | - |
? | |
| 4.1.2.47 | HCN + pentanal | - |
Manihot esculenta | 2-hydroxyhexanenitrile | - |
? | |
| 4.1.2.47 | HCN + propanal | - |
Manihot esculenta | 2-hydroxybutanenitrile | - |
? | |
| 4.1.2.47 | HCN + thiophen-2-carbaldehyde | - |
Manihot esculenta | hydroxy(thien-2-yl)acetonitrile | - |
? | |
| 4.1.2.47 | HCN + thiophen-3-carbaldehyde | - |
Manihot esculenta | hydroxy(thien-3-yl)acetonitrile | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.2.10 | monomer | 1 * 55000-80000 | Prunus sp. |
| 4.1.2.11 | ? | x * 33000 + x * 22000 | Sorghum bicolor |
| 4.1.2.47 | dimer | 2 * 42000 | Linum usitatissimum |
| 4.1.2.47 | polymer | x * 28000-30000 | Manihot esculenta |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.2.10 | Hydroxynitrile lyase | - |
Prunus sp. |
| 4.1.2.11 | (S)-HNL | - |
Sorghum bicolor |
| 4.1.2.11 | (S)-SbHNL | - |
Sorghum bicolor |
| 4.1.2.11 | Hydroxynitrile lyase | - |
Sorghum bicolor |
| 4.1.2.47 | (R)-LuHNL | - |
Linum usitatissimum |
| 4.1.2.47 | (R)-Oxynitrilase | - |
Linum usitatissimum |
| 4.1.2.47 | (S)-HNL | - |
Manihot esculenta |
| 4.1.2.47 | (S)-MeHNL | - |
Manihot esculenta |
| 4.1.2.47 | HNL | - |
Linum usitatissimum |
| 4.1.2.47 | HNL | - |
Manihot esculenta |
| 4.1.2.47 | Hydroxynitrile lyase | - |
Linum usitatissimum |
| 4.1.2.47 | Hydroxynitrile lyase | - |
Manihot esculenta |
| 4.1.2.47 | LuHNL | - |
Linum usitatissimum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.10 | 5 | 6 | - |
Prunus sp. |
| 4.1.2.47 | 5.4 | - |
- |
Manihot esculenta |
| 4.1.2.47 | 5.5 | - |
- |
Linum usitatissimum |
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Release 2023.1 (January 2023)
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