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Literature summary extracted from

  • Cho, J.S.; Lee, C.W.; Kang, S.H.; Lee, J.C.; Bok, J.D.; Moon, Y.S.; Lee, H.G.; Kim, S.C.; Choi, Y.J.
    Purification and characterization of a phytase from Pseudomonas syringae MOK1 (2003), Curr. Microbiol., 47, 290-294.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Cd2+ 5 mM, 70% inhibition Pseudomonas syringae
3.1.3.8 Cu2+ 5 mM, 70% inhibition Pseudomonas syringae
3.1.3.8 EDTA 5 mM, 70% inhibition Pseudomonas syringae
3.1.3.8 Mn2+ 5 mM, 70% inhibition Pseudomonas syringae
3.1.3.26 Cd2+ 5 mM, 70% inhibition Pseudomonas syringae
3.1.3.26 Cu2+ 5 mM, 70% inhibition Pseudomonas syringae
3.1.3.26 EDTA 5 mM, 70% inhibition Pseudomonas syringae
3.1.3.26 Mn2+ 5 mM, 70% inhibition Pseudomonas syringae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.38
-
 myo-inositol hexakisphosphate pH 5.5, 40°C Pseudomonas syringae
3.1.3.26 0.38
-
 myo-inositol hexakisphosphate pH 5.5, 40°C Pseudomonas syringae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 45000
-
 x * 45000, SDS-PAGE Pseudomonas syringae
3.1.3.26 45000
-
 x * 45000, SDS-PAGE Pseudomonas syringae

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Pseudomonas syringae
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Pseudomonas syringae MOK1
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Pseudomonas syringae
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Pseudomonas syringae MOK1
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
 Pseudomonas syringae
3.1.3.26
-
 Pseudomonas syringae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O very specific for phytate Pseudomonas syringae ? + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O very specific for phytate Pseudomonas syringae MOK1 ? + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O very specific for phytate Pseudomonas syringae ? + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O very specific for phytate Pseudomonas syringae MOK1 ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 45000, SDS-PAGE Pseudomonas syringae
3.1.3.26 ? x * 45000, SDS-PAGE Pseudomonas syringae

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 MOK1 phytase
-
 Pseudomonas syringae
3.1.3.26 MOK1 phytase
-
 Pseudomonas syringae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 40
-
-
 Pseudomonas syringae
3.1.3.26 40
-
-
 Pseudomonas syringae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 20 50 20°C: about 30% of maximal activity, 50°C: about 45% of maximal activity Pseudomonas syringae
3.1.3.26 20 50 20°C: about 30% of maximal activity, 50°C: about 45% of maximal activity Pseudomonas syringae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5.5
-
-
 Pseudomonas syringae
3.1.3.26 5.5
-
-
 Pseudomonas syringae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 4.2 6.5 pH 4.2: about 80% of maximal activity, pH 6.5: about 70% of maximal activity Pseudomonas syringae
3.1.3.26 4.2 6.5 pH 4.2: about 80% of maximal activity, pH 6.5: about 70% of maximal activity Pseudomonas syringae