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Literature summary extracted from

  • Virto, C.; Svensson, I.; Adlercreutz, P.
    Hydrolytic and transphosphatidylation activities of phospholipase D from Savoy cabbage towards lysophosphatidylcholine (2000), Chem. Phys. Lipids, 106, 41-51.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 CTAB inhibitory at concentrations at or above that of 1 lysophosphatidylcholine Brassica oleracea var. capitata
3.1.4.4 SDS inhibitory at concentrations at or above that of 1 lysophosphatidylcholine Brassica oleracea var. capitata
3.1.4.4 Triton X-100 inhibitory at concentrations at or above that of 1 lysophosphatidylcholine Brassica oleracea var. capitata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 1
-
 lysophosphatidylcholine at 4 mM Ca2+ Brassica oleracea var. capitata
3.1.4.4 3
-
 lysophosphatidylcholine at 40 mM Ca2+ Brassica oleracea var. capitata
3.1.4.4 388
-
 glycerol transphosphatidylation Brassica oleracea var. capitata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ absolute requirement, optimal at 40 mM Brassica oleracea var. capitata

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.4.4 Glycerol for measurement of transfer reaction, 2 M included in media to suppress hydrolytic reaction Brassica oleracea var. capitata

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Brassica oleracea var. capitata
-
 var. sabauda
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 lysophosphatidylcholine + glycerol
-
 Brassica oleracea var. capitata ?
-
 ?
3.1.4.4 lysophosphatidylcholine + H2O about 20 times slower than reaction of phosphatidylcholine Brassica oleracea var. capitata choline + lysophosphatidate
-
 ?
3.1.4.4 phosphatidylcholine + glycerol
-
 Brassica oleracea var. capitata ?
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Brassica oleracea var. capitata choline + phosphatidate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 5.5
-
-
 Brassica oleracea var. capitata