BRENDA - Enzyme Database

Chemical modification of lipases with various hydrophobic groups improves their enantioselectivity in hydrolytic reactions

Ueji, S.i.; Ueda, A.; Tanaka, H.; Watanabe, K.; Okamoto, T.; Ebara, Y.; Biotechnol. Lett. 25, 83-87 (2003)

Data extracted from this reference:

Engineering
EC Number
Protein Variants
Commentary
Organism
3.1.1.3
additional information
lipase is modified by substitution with diverse side chain groups, overview, lipase MY modified with a benzyloxycarbonyl group, modified enzyme shows reduced activity and 15fold increased enantioselectivity
Diutina rugosa
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.1.3
Diutina rugosa
-
lipase MY modified with a benzyloxycarbonyl group
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.1.3
racemic butyl 2-(4-ethylphenoxy)propionate + H2O
modified enzyme shows reduced activity and 15fold increased enantioselectivity
650697
Diutina rugosa
2-(4-ethylphenoxy)propionate + butanol
-
650697
Diutina rugosa
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.1.1.3
lipase MY
-
Diutina rugosa
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.1.3
37
-
assay at
Diutina rugosa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.3
6.9
-
assay at
Diutina rugosa
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
3.1.1.3
additional information
lipase is modified by substitution with diverse side chain groups, overview, lipase MY modified with a benzyloxycarbonyl group, modified enzyme shows reduced activity and 15fold increased enantioselectivity
Diutina rugosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.1.3
racemic butyl 2-(4-ethylphenoxy)propionate + H2O
modified enzyme shows reduced activity and 15fold increased enantioselectivity
650697
Diutina rugosa
2-(4-ethylphenoxy)propionate + butanol
-
650697
Diutina rugosa
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.1.3
37
-
assay at
Diutina rugosa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.3
6.9
-
assay at
Diutina rugosa