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Literature summary extracted from

  • Kim, H.W.; Kim, Y.O.; Lee, J.H.; Kim, K.K.; Kim, Y.J.
    Isolation and characterization of a phytase with improved properties from Citrobacter braakii (2003), Biotechnol. Lett., 25, 1231-1234.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.8 Triton X-100 enhances activity Citrobacter braakii
3.1.3.26 Triton X-100 enhances activity Citrobacter braakii

General Stability

EC Number General Stability Organism
3.1.3.8 the enzyme is resistant to proteases such as trypsin or pepsin when incubated for 2 h at 37°C. Papain, elastase and pancreatin slightly decrease activity to 85%, 80% and 70%, respectively, of the original activity Citrobacter braakii
3.1.3.26 the enzyme is resistant to proteases such as trypsin or pepsin when incubated for 2 h at 37°C. Papain, elastase and pancreatin slightly decrease activity to 85%, 80% and 70%, respectively, of the original activity Citrobacter braakii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Cu2+
-
 Citrobacter braakii
3.1.3.8 Fe3+
-
 Citrobacter braakii
3.1.3.8 additional information not affected at concentrations up to 10 mM by: EDTA, Cd2+, Ca2+, K+, Mg2+, Mn2+, Ni2+ and Na+ Citrobacter braakii
3.1.3.8 SDS 0.0025%, complete inactivation Citrobacter braakii
3.1.3.8 Urea 8 M, complete inactivation Citrobacter braakii
3.1.3.8 Zn2+
-
 Citrobacter braakii
3.1.3.26 Cu2+
-
 Citrobacter braakii
3.1.3.26 Fe3+
-
 Citrobacter braakii
3.1.3.26 additional information not affected at concentrations up to 10 mM by: EDTA, Cd2+, Ca2+, K+, Mg2+, Mn2+, Ni2+ and Na+ Citrobacter braakii
3.1.3.26 SDS 0.0025%, complete inactivation Citrobacter braakii
3.1.3.26 Urea 8 M, complete inactivation Citrobacter braakii
3.1.3.26 Zn2+
-
 Citrobacter braakii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.46
-
 myo-inositol hexakisphosphate pH 4, 37°C Citrobacter braakii
3.1.3.26 0.46
-
 myo-inositol hexakisphosphate pH 4, 37°C Citrobacter braakii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 intracellular
-
 Citrobacter braakii 5622
-
3.1.3.26 intracellular
-
 Citrobacter braakii 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 additional information not affected at concentrations up to 10 mM by: Cd2+, Ca2+, K+, Mg2+, Mn2+, Ni2+ and Na+ Citrobacter braakii
3.1.3.26 additional information not affected at concentrations up to 10 mM by: Cd2+, Ca2+, K+, Mg2+, Mn2+, Ni2+ and Na+ Citrobacter braakii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 47000
-
 x * 47000, SDS-PAGE Citrobacter braakii
3.1.3.26 47000
-
 x * 47000, SDS-PAGE Citrobacter braakii

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Citrobacter braakii
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.8 Citrobacter braakii YH-15
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Citrobacter braakii
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-
3.1.3.26 Citrobacter braakii YH-15
-
 The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
 Citrobacter braakii
3.1.3.26
-
 Citrobacter braakii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 3457
-
-
 Citrobacter braakii
3.1.3.26 3457
-
-
 Citrobacter braakii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 4-nitrophenyl phosphate + H2O 11% of the activity with myo-inositol hexakisphosphate Citrobacter braakii 4-nitrophenol + phosphate
-
 ?
3.1.3.8 additional information less than 5% of the activity with myo-inositol hexakisphosphate with: ATP, ADP, glycerophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate and mannose 6-phosphate Citrobacter braakii ?
-
 ?
3.1.3.8 additional information less than 5% of the activity with myo-inositol hexakisphosphate with: ATP, ADP, glycerophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate and mannose 6-phosphate Citrobacter braakii YH-15 ?
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Citrobacter braakii ? + phosphate
-
 ?
3.1.3.8 tetrasodium diphosphate + H2O 6% of the activity with myo-inositol hexakisphosphate Citrobacter braakii ?
-
 ?
3.1.3.26 4-nitrophenyl phosphate + H2O 11% of the activity with myo-inositol hexakisphosphate Citrobacter braakii 4-nitrophenol + phosphate
-
 ?
3.1.3.26 4-nitrophenyl phosphate + H2O 11% of the activity with myo-inositol hexakisphosphate Citrobacter braakii YH-15 4-nitrophenol + phosphate
-
 ?
3.1.3.26 additional information less than 5% of the activity with myo-inositol hexakisphosphate with: ATP, ADP, glycerophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate and mannose 6-phosphate Citrobacter braakii ?
-
 ?
3.1.3.26 additional information less than 5% of the activity with myo-inositol hexakisphosphate with: ATP, ADP, glycerophosphate, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate and mannose 6-phosphate Citrobacter braakii YH-15 ?
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Citrobacter braakii ? + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Citrobacter braakii YH-15 ? + phosphate
-
 ?
3.1.3.26 tetrasodium diphosphate + H2O 6% of the activity with myo-inositol hexakisphosphate Citrobacter braakii ?
-
 ?
3.1.3.26 tetrasodium diphosphate + H2O 6% of the activity with myo-inositol hexakisphosphate Citrobacter braakii YH-15 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 47000, SDS-PAGE Citrobacter braakii
3.1.3.26 ? x * 47000, SDS-PAGE Citrobacter braakii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 50
-
-
 Citrobacter braakii
3.1.3.26 50
-
-
 Citrobacter braakii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.8 25 60 25°C: about 45% of maximal activity, 60°C: about 35% of maximal activity Citrobacter braakii
3.1.3.26 25 60 25°C: about 45% of maximal activity, 60°C: about 35% of maximal activity Citrobacter braakii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 50
-
 pH 4, 30 min, stable up to Citrobacter braakii
3.1.3.8 55
-
 pH 4, 30 min, about 65% loss of activity Citrobacter braakii
3.1.3.8 60
-
 pH 4, 30 min, about 90% loss of activity Citrobacter braakii
3.1.3.8 65
-
 pH 4, 30 min, about 95% loss of activity Citrobacter braakii
3.1.3.26 50
-
 pH 4, 30 min, stable up to Citrobacter braakii
3.1.3.26 55
-
 pH 4, 30 min, about 65% loss of activity Citrobacter braakii
3.1.3.26 60
-
 pH 4, 30 min, about 90% loss of activity Citrobacter braakii
3.1.3.26 65
-
 pH 4, 30 min, about 95% loss of activity Citrobacter braakii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 4
-
-
 Citrobacter braakii
3.1.3.26 4
-
-
 Citrobacter braakii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 2.5 5.5 about 50% of maximal activity at pH 2.5 and pH 5.5 Citrobacter braakii
3.1.3.26 2.5 5.5 about 50% of maximal activity at pH 2.5 and pH 5.5 Citrobacter braakii