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Literature summary extracted from

  • Oh, S.W.; Gaskin, D.J.H.; Kwon, D.Y.; Vulfson, E.N.
    Properties of recombinant Rhizomucor miehei lipase with amino acid substitutions of Phe94 in the substrate binding domain (2001), Biotechnol. Lett., 23, 563-568.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 subcloning of plasmids encoding mutant enzymes in Escherichia coli XLIB, expression of mutants in Pichia pastoris GS115 Rhizomucor miehei

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 F94E site-directed mutagenesis, altered substrate chain length specificity Rhizomucor miehei
3.1.1.3 F94Q site-directed mutagenesis, altered substrate chain length specificity Rhizomucor miehei
3.1.1.3 F94R site-directed mutagenesis, altered substrate chain length specificity Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Rhizomucor miehei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 preparative level, recombinant mutants expressed in Pichia pastoris Rhizomucor miehei

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.3 triacylglycerol + H2O = diacylglycerol + a carboxylate Phe94 is involved in substrate binding and is responsible for accommodating the acyl chain length of the substrate Rhizomucor miehei
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 1,2-O-dilauryl-rac-glycero-3-glutaric acid resorufin ester + H2O
-
 Rhizomucor miehei ?
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O low activity Rhizomucor miehei 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl decanoate + H2O
-
 Rhizomucor miehei 4-nitrophenol + decanoate
-
 ?
3.1.1.3 4-nitrophenyl hexanoate + H2O low activity Rhizomucor miehei 4-nitrophenol + hexanoate
-
 ?
3.1.1.3 4-nitrophenyl octanoate + H2O low activity Rhizomucor miehei 4-nitrophenol + octanoate
-
 ?
3.1.1.3 4-nitrophenyl tetradecanoate + H2O best 4-nitrophenyl ester substrate for wild-type enzyme and mutant F94R Rhizomucor miehei 4-nitrophenol + tetradecanoate
-
 ?
3.1.1.3 additional information substrate chain length specificity of wild-type and mutant enzymes Rhizomucor miehei ?
-
 ?
3.1.1.3 tributyrin + H2O best triacylglyceride substrate for the wild-type enzyme Rhizomucor miehei dibutyrin + butyrate
-
 ?
3.1.1.3 tridecanin + H2O
-
 Rhizomucor miehei didecanin + decanoate
-
 ?
3.1.1.3 trihexanin + H2O
-
 Rhizomucor miehei dihexanin + hexanoate
-
 ?
3.1.1.3 trioctanin + H2O
-
 Rhizomucor miehei dioctanin + octanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Rhizomucor miehei