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Literature summary extracted from

  • Rathi, P.; Bradoo, S.; Saxena, R.K.; Gupta, R.
    A hyper-thermostable, alkaline lipase from Pseudomonas sp. with the property of thermal activation (2000), Biotechnol. Lett., 22, 495-498.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 additional information
-
 additional information
-
 Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas sp.
-
 conatians a hyper-thermostable lipase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 partial, 6.8fold Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 167
-
 partially purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 castor oil + H2O major acyl group is ricinoleic acid Pseudomonas sp. ?
-
 ?
3.1.1.3 coconut oil + H2O major acyl group is palmitic acid Pseudomonas sp. ?
-
 ?
3.1.1.3 corn oil + H2O major acyl groups are oleic acid and linoleic acid Pseudomonas sp. ?
-
 ?
3.1.1.3 groundnut oil + H2O major acyl group is oleic acid Pseudomonas sp. ?
-
 ?
3.1.1.3 linseed oil + H2O major acyl groups are oleic and linoleic acid Pseudomonas sp. ?
-
 ?
3.1.1.3 mustard oil + H2O major acyl groups are erucic and oleic acid Pseudomonas sp. ?
-
 ?
3.1.1.3 neem oil + H2O major acyl groups are palmitic, stearic and oleic acid Pseudomonas sp. ?
-
 ?
3.1.1.3 olive oil + H2O best substrate, major acyl group is oleic acid Pseudomonas sp. ?
-
 ?
3.1.1.3 soybean oil + H2O major acyl groups are oleic acid and linoleic acid Pseudomonas sp. ?
-
 ?
3.1.1.3 sunflower oil + H2O major acyl group is linoleic acid Pseudomonas sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 90
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 90
-
 8 h 100% stable, 13 h loss of 50% activity, after 2 h at 90°C 30% activation is observed Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 11
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 9 12
-
 Pseudomonas sp.