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Literature summary extracted from

  • Ignatova, Z.; Stoeva, S.; Galunsky, B.; Hörnle, C.; Nurk, A.; Piotraschke, E.; Voelter, W.; Kasche, V.
    Proteolytic processing of penicillin amidase from Alcaligenes faecalis cloned in Escherichia coli yields several active forms (1998), Biotechnol. Lett., 20, 977-982.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.11 expression in Escherichia coli Alcaligenes faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.11 0.008
-
 benzylpenicillin pH 7.5, 25°C, enzyme form PA5..5 and PA5.3 Alcaligenes faecalis
3.5.1.11 0.01
-
 benzylpenicillin pH 7.5, 25°C Escherichia coli
3.5.1.11 0.012
-
 6-nitro-3-phenylacetamidobenzoic acid pH 7.5, 25°C, enzyme form PA5.3 Alcaligenes faecalis
3.5.1.11 0.017
-
 6-nitro-3-phenylacetamidobenzoic acid pH 7.5, 25°C, enzyme form PA5.5 Alcaligenes faecalis
3.5.1.11 0.02
-
 6-nitro-3-phenylacetamidobenzoic acid pH 7.5, 25°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.11 22540
-
 ? * 22540 + ? * 62568, enzyme form PA5.3, A chain and B-chain MALDI Alcaligenes faecalis
3.5.1.11 22920
-
 ? * 22920 + ? * 62564, enzyme form PA5.5, A chain and B-chain MALDI Alcaligenes faecalis
3.5.1.11 62564
-
 ? * 22920 + ? * 62564, enzyme form PA5.5, A chain and B-chain MALDI Alcaligenes faecalis
3.5.1.11 62568
-
 ? * 22540 + ? * 62568, enzyme form PA5.3, A chain and B-chain MALDI Alcaligenes faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Alcaligenes faecalis
-
-
-
3.5.1.11 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.11 recombinant enzyme expressed in Escherichia coli Alcaligenes faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.11 additional information
-
 3.8 DELTAA380/min per absorbance of the enzyme solution at 280 nM for enzyme form PA5.3 and 3.2 DELTAA380/min per absorbance of the enzyme solution at 280 nM for enzyme form PA5.5 Alcaligenes faecalis

Storage Stability

EC Number Storage Stability Organism
3.5.1.11 4 °C, half-life is 6 h Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 6-nitro-3-phenylacetamidobenzoic acid + H2O
-
 Alcaligenes faecalis ?
-
 ?
3.5.1.11 6-nitro-3-phenylacetamidobenzoic acid + H2O
-
 Escherichia coli ?
-
 ?
3.5.1.11 benzylpenicillin + H2O
-
 Alcaligenes faecalis phenylacetic acid + 6-aminopenicillanate
-
 ?
3.5.1.11 benzylpenicillin + H2O
-
 Escherichia coli phenylacetic acid + 6-aminopenicillanate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.11 ? ? * 22540 + ? * 62568, enzyme form PA5.3, A chain and B-chain MALDI Alcaligenes faecalis
3.5.1.11 ? ? * 22920 + ? * 62564, enzyme form PA5.5, A chain and B-chain MALDI Alcaligenes faecalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.11 4
-
 half-life: 6 h Alcaligenes faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.11 20
-
 6-nitro-3-phenylacetamidobenzoic acid pH 7.5, 25°C Escherichia coli
3.5.1.11 50
-
 benzylpenicillin pH 7.5, 25°C Escherichia coli
3.5.1.11 75
-
 benzylpenicillin pH 7.5, 25°C, enzyme form PA5..5 Alcaligenes faecalis
3.5.1.11 77
-
 6-nitro-3-phenylacetamidobenzoic acid pH 7.5, 25°C, enzyme form PA5..5 Alcaligenes faecalis
3.5.1.11 80
-
 benzylpenicillin pH 7.5, 25°C, enzyme form PA5..3 Alcaligenes faecalis
3.5.1.11 82
-
 6-nitro-3-phenylacetamidobenzoic acid pH 7.5, 25°C, enzyme form PA5..3 Alcaligenes faecalis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.11 Alcaligenes faecalis enzyme form PA5.3, isoelectric focusing
-
 5.3
3.5.1.11 Alcaligenes faecalis enzyme form PA5.5, isoelectric focusing
-
 5.5
3.5.1.11 Escherichia coli isoelectric focusing
-
 7