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Literature summary extracted from

  • Piotraschke, E.; Nurk, A.; Galunsky, B.; Kasche, V.
    Genetic construction of catalytically active cross-species heterodimer penicillin G amidase (1994), Biotechnol. Lett., 16, 119-124.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.11 a cross-species penicillin G amidase gene coding for an alpha-peptide and a linker peptide from K. citrophila and a beta-peptide from Escherichia coli constructed and cloned in Escherichia coli Escherichia coli
3.5.1.11 a cross-species penicillin G amidase gene coding for an alpha-peptide and a linker peptide from Kluyvera citrophila and a beta-peptide from Escherichia coli constructed and cloned in Escherichia coli Kluyvera cryocrescens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.11 additional information a cross-species penicillin G amidase gene coding for an alpha-peptide and a linker peptide from K. citrophila and a beta-peptide from Escherichia coli constructed and cloned in Escherichia coli. In comparison with the two wild-type enzymes the hybrid enzyme has a higher turnover-number for benzylpenicillin, ampicillin and 6-nitro-3-phenylacetamido-benzoic acid. The KM-values are between the values of the wild-type enzymes or close ro that of K. citrophila Escherichia coli
3.5.1.11 additional information a cross-species penicillin G amidase gene coding for an alpha-peptide and a linker peptide from Kluyvera citrophila and a beta-peptide from Escherichia coli constructed and cloned in Escherichia coli. In comparison with the two wild-type enzymes the hybrid enzyme has a higher turnover-number for benzylpenicillin, ampicillin and 6-nitro-3-phenylacetamido-benzoic acid. The Km-values are between the values of the wild-type enzymes or close ro that of Kluyvera citrophila Kluyvera cryocrescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.11 0.005
-
benzylpenicillin pH 7.5, 25°C Escherichia coli
3.5.1.11 0.018
-
benzylpenicillin pH 7.5, 25°C Kluyvera cryocrescens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Escherichia coli
-
ATCC 11105
-
3.5.1.11 Kluyvera cryocrescens
-
ATCC 21285
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 6-nitro-3-phenylacetamido-benzoic acid + H2O
-
Escherichia coli ?
-
?
3.5.1.11 6-nitro-3-phenylacetamido-benzoic acid + H2O
-
Kluyvera cryocrescens ?
-
?
3.5.1.11 ampicillin + H2O
-
Escherichia coli ?
-
?
3.5.1.11 ampicillin + H2O
-
Kluyvera cryocrescens ?
-
?
3.5.1.11 benzylpenicillin + H2O
-
Escherichia coli phenylacetic acid + 6-aminopenicillanate
-
?
3.5.1.11 benzylpenicillin + H2O
-
Kluyvera cryocrescens phenylacetic acid + 6-aminopenicillanate
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.11 50
-
benzylpenicillin pH 7.5, 25°C Escherichia coli
3.5.1.11 56.5
-
benzylpenicillin pH 7.5, 25°C Kluyvera cryocrescens