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Literature summary extracted from

  • Arreguin-Espinosa, R.; Arreguin, B.; Gonzalez, C.
    Purification and properties of a lipase from Cephaloleia presignis (Coleoptera, Chrysomelidae) (2000), Biotechnol. Appl. Biochem., 31, 239-244.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 additional information not affected by Ca2+, Mn2+, Mg2+, Na+, K+, PMSF, EDTA, all at 1 mM Cephaloleia presignis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Ag+ complete inhibition, 1 mM Cephaloleia presignis
3.1.1.3 Cu2+ complete inhibition, 1 mM Cephaloleia presignis
3.1.1.3 diisopropyl fluorophosphate complete inhibition, 1 mM Cephaloleia presignis
3.1.1.3 Fe2+ complete inhibition, 1 mM Cephaloleia presignis
3.1.1.3 Fe3+ complete inhibition, 1 mM Cephaloleia presignis
3.1.1.3 additional information not affected by Ca2+, Mn2+, Mg2+, Na+, K+, PMSF, EDTA, all at 1 mM Cephaloleia presignis
3.1.1.3 Zn2+ complete inhibition, 1 mM Cephaloleia presignis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular hemolymph Cephaloleia presignis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 additional information no metalloenzyme Cephaloleia presignis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 29000
-
 gel filtration Cephaloleia presignis
3.1.1.3 31000
-
 1 * 31000, SDS-PAGE Cephaloleia presignis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Cephaloleia presignis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 glycoprotein
-
 Cephaloleia presignis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 20.3fold Cephaloleia presignis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 hemolymph
-
 Cephaloleia presignis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 13.26
-
 purified enzyme Cephaloleia presignis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl acetate + H2O low activity Cephaloleia presignis 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O low activity Cephaloleia presignis 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Cephaloleia presignis 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl oleate + H2O
-
 Cephaloleia presignis 4-nitrophenol + oleate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O high activity Cephaloleia presignis 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl propionate + H2O
-
 Cephaloleia presignis 4-nitrophenol + propionate
-
 ?
3.1.1.3 alpha-naphthyl acetate + H2O
-
 Cephaloleia presignis alpha-naphthol + acetate
-
 ?
3.1.1.3 methyl acetate + H2O
-
 Cephaloleia presignis methanol + acetate
-
 ?
3.1.1.3 methyl butyrate + H2O
-
 Cephaloleia presignis methanol + butyrate
-
 ?
3.1.1.3 methyl laurate + H2O
-
 Cephaloleia presignis methanol + laurate
-
 ?
3.1.1.3 methyl oleate + H2O high activity Cephaloleia presignis methanol + oleate
-
 ?
3.1.1.3 methyl palmitate + H2O high activity Cephaloleia presignis methanol + palmitate
-
 ?
3.1.1.3 methyl propionate + H2O
-
 Cephaloleia presignis methanol + propionate
-
 ?
3.1.1.3 methyl stearate + H2O
-
 Cephaloleia presignis methanol + stearate
-
 ?
3.1.1.3 additional information olive oil is utilized as substrate with high activity, the enzyme hydrolyzes long-chain fatty acids rather than short-chain fatty acids of 4-nitrophenyl esters and methyl esters, and it hydrolyzes short-chain fatty acids rather than short-shain fatty acids of triglycerides Cephaloleia presignis ?
-
 ?
3.1.1.3 tributyrin + H2O best substrate Cephaloleia presignis dibutyrin + butyrate
-
 ?
3.1.1.3 trilaurin + H2O high activity Cephaloleia presignis dilaurin + laurate
-
 ?
3.1.1.3 triolein + H2O
-
 Cephaloleia presignis diolein + oleate
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Cephaloleia presignis dipalmitin + palmitate
-
 ?
3.1.1.3 tripropionin + H2O
-
 Cephaloleia presignis dipropionin + propionate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 monomer 1 * 31000, SDS-PAGE Cephaloleia presignis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Cephaloleia presignis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7
-
-
 Cephaloleia presignis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Cephaloleia presignis isoelectric focusing
-
 6.9